Tezuka K, Takeshita S, Hakeda Y, Kumegawa M, Kikuno R, Hashimoto-Gotoh T
Pharma Research Laboratories, Hoechst Japan Ltd., Kawagoe.
Biochem Biophys Res Commun. 1990 Nov 30;173(1):246-51. doi: 10.1016/s0006-291x(05)81048-4.
Using the differential hybridization screening method between osteoblastic and fibroblastic cells, a cDNA clone coding for an osteoblast specific protein, named OSF-1, consisting of 168 amino acid residues including a possible 32 amino acid long leader sequence, was isolated from murine osteoblastic cell line MC3T3-E1. The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblast-enriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. The human counterpart was also found in cDNA libraries from human osteosarcoma cell line MG63 and normal brain tissues. DNA sequence analysis revealed four amino acid sequence differences between the mouse and human, of which only one is located in the mature protein. This extremely high sequence conservation suggests that OSF-1 plays a fundamental role in bone and brain functions.
利用成骨细胞与成纤维细胞之间的差异杂交筛选方法，从鼠成骨细胞系MC3T3-E1中分离出一个编码成骨细胞特异性蛋白的cDNA克隆，命名为OSF-1，它由168个氨基酸残基组成，包括一个可能长达32个氨基酸的前导序列。通过Northern印迹分析表明，OSF-1基因在富含小鼠颅骨成骨细胞的细胞和小鼠脑组织中表达，但在胸腺、脾脏、肾脏、肝脏、肺、睾丸或心脏中不表达。在人骨肉瘤细胞系MG63和正常脑组织的cDNA文库中也发现了其人类对应物。DNA序列分析揭示了小鼠和人类之间存在四个氨基酸序列差异，其中只有一个位于成熟蛋白中。这种极高的序列保守性表明OSF-1在骨骼和大脑功能中起重要作用。
