Bouderlique Thibault, Henault Emilie, Lebouvier Angelique, Frescaline Guilhem, Bierling Phillipe, Rouard Helene, Courty José, Albanese Patricia, Chevallier Nathalie
CNRS EAC 7149, CRRET Laboratory, Paris-Est University, Créteil, France ; EA3952, Cellular and Tissular Bioengineering Laboratory, Paris-Est University, Créteil, France.
CNRS EAC 7149, CRRET Laboratory, Paris-Est University, Créteil, France.
PLoS One. 2014 Feb 7;9(2):e88287. doi: 10.1371/journal.pone.0088287. eCollection 2014.
Pleiotrophin (PTN) is a growth factor present in the extracellular matrix of the growth plate during bone development and in the callus during bone healing. Bone healing is a complicated process that recapitulates endochondral bone development and involves many cell types. Among those cells, mesenchymal stromal cells (MSC) are able to differentiate toward chondrogenic and osteoblastic lineages. We aimed to determine PTN effects on differentiation properties of human bone marrow stromal cells (hBMSC) under chondrogenic induction using histological analysis and quantitative reverse transcription polymerase chain reaction. PTN dramatically potentiated chondrogenic differentiation as indicated by a strong increase of collagen 2 protein, and cartilage-related gene expression. Moreover, PTN increased transcription of hypertrophic chondrocyte markers such as MMP13, collagen 10 and alkaline phosphatase and enhanced calcification and the content of collagen 10 protein. These effects are dependent on PTN receptors signaling and PI3 K pathway activation. These data suggest a new role of PTN in bone regeneration as an inducer of hypertrophy during chondrogenic differentiation of hBMSC.
多效生长因子（PTN）是一种生长因子，在骨骼发育过程中存在于生长板的细胞外基质中，在骨愈合过程中存在于骨痂中。骨愈合是一个复杂的过程，它重现了软骨内成骨发育，涉及多种细胞类型。在这些细胞中，间充质基质细胞（MSC）能够向软骨生成和成骨谱系分化。我们旨在通过组织学分析和定量逆转录聚合酶链反应，确定PTN对软骨生成诱导下人骨髓基质细胞（hBMSC）分化特性的影响。如胶原蛋白2蛋白和软骨相关基因表达的强烈增加所示，PTN显著增强了软骨生成分化。此外，PTN增加了肥大软骨细胞标志物如MMP13、胶原蛋白10和碱性磷酸酶的转录，并增强了钙化和胶原蛋白10蛋白的含量。这些作用依赖于PTN受体信号传导和PI3 K途径激活。这些数据表明PTN在骨再生中作为hBMSC软骨生成分化过程中肥大的诱导剂具有新的作用。
