一种用于测量氟化银和碘化钾处理对脱矿牙本质对变形链球菌渗透性影响的体外模型。

An in vitro model to measure the effect of a silver fluoride and potassium iodide treatment on the permeability of demineralized dentine to Streptococcus mutans.

作者信息

Knight G M, McIntyre J M, Craig G G, Zilm P S, Gully N J

机构信息

Dental School, Faculty of Health Sciences, The University of Adelaide.

出版信息

Aust Dent J. 2005 Dec;50(4):242-5. doi: 10.1111/j.1834-7819.2005.tb00367.x.

DOI:10.1111/j.1834-7819.2005.tb00367.x

PMID:17016889

Abstract

BACKGROUND

Diamine silver fluoride (Ag(NH3)2F), referred to as AgF, has been used to reduce the incidence of caries in primary dentitions but has been limited by the associated staining of both teeth and restorative materials. The application of potassium iodide (KI), following AgF prevents staining but its effects on the ability of AgF to reduce caries are not known. The aim of this study was to develop an in vitro model that would provide an indication of the permeability of demineralized dentine to Streptococcus mutans after treatment of the dentine with AgF followed by KI.

METHODS

Forty dentine discs were bonded to the base of forty 5mL polycarbonate screw top vials (that had had their bases removed), filled with nutrient medium, sterilized and placed into a continuous culture of S. mutans. Samples were divided into four groups as follows: 10 samples of demineralized dentine as a control, 10 samples of demineralized dentine treated with AgF/KI, 10 samples of demineralized dentine treated with KI and 10 samples of demineralized dentine treated with AgF. After two weeks the optical density of the growth medium chambers was measured to determine bacterial penetration and growth. Cultures were plated out to determine migration through the discs by S. mutans.

RESULTS

S. mutans migrated through all dentine discs. However, the samples treated with AgF and AgF/KI had significantly lower optical densities than the corresponding controls. The range of optical densities was least amongst demineralized samples treated with AgF/KI.

CONCLUSIONS

Under the conditions of this study, treatment of demineralized dentine discs with AgF followed by KI allowed the penetration of S. mutans. Based on optical density measurements, the treatment resulted in significantly fewer microorganisms being present subjacent to the discs treated with AgF and KI than the control discs at the end of the experimental period.

摘要

背景

二胺氟化银（Ag(NH3)2F），简称为AgF，已被用于降低乳牙龋齿的发生率，但受到牙齿和修复材料相关染色的限制。在AgF之后应用碘化钾（KI）可防止染色，但其对AgF降低龋齿能力的影响尚不清楚。本研究的目的是建立一种体外模型，以表明在用AgF然后用KI处理牙本质后，脱矿牙本质对变形链球菌的渗透性。

方法

将40个牙本质圆盘粘结到40个5毫升聚碳酸酯螺旋盖小瓶（已去除底部）的底部，装满营养培养基，灭菌并放入变形链球菌的连续培养物中。样品分为四组如下：10个脱矿牙本质样品作为对照，10个用AgF/KI处理的脱矿牙本质样品，10个用KI处理的脱矿牙本质样品和10个用AgF处理的脱矿牙本质样品。两周后，测量生长培养基腔室的光密度以确定细菌的渗透和生长。将培养物接种以确定变形链球菌通过圆盘的迁移情况。