Wang Licong, Cao Yuhua, Xu Honglan, Ye Jiannong
School of Chemical and Material Engineering, Southern Yangze University, Wuxi 214036, China.
Se Pu. 2006 Jul;24(4):367-72.
The chromatographic fingerprint of Chinese traditional medicine, Yinhuang Oral Liquid together with its original materials Radix Scutellariae and Flos lonicera was established by high performance liquid chromatography (HPLC). At the same time, baicalin and chlorogenic acid in the Yinhuang Oral Liquid were also quantitatively determined. The analysis was performed on a Lichrospher C18 column (250 mm x 4.6 mm i. d. , 5 microm) and with gradient elution. The optimum conditions were explored. The mobile phase A was methanol and the mobile phase B was 0.1% H3PO4 aqueous solution. The wavelength of 254 nm was selected for detection. Finally, the data of fingerprints of 6 samples of Yinhuang Oral Liquid were processed with two different mathematic methods. This proposed method demonstrated good stability for the 6 samples of Yinhuang Oral Liquid. In the comparison of the common peaks in chromatogram of Yinhuang Oral Liquid with those of crude herbs, most of them could be matched well.
采用高效液相色谱法（HPLC）建立了中药银黄口服液及其原料药黄芩和金银花的色谱指纹图谱。同时，对银黄口服液中的黄芩苷和绿原酸进行了定量测定。分析在Lichrospher C18柱（250 mm×4.6 mm内径，5μm）上进行，采用梯度洗脱。探索了最佳条件。流动相A为甲醇，流动相B为0.1% H3PO4水溶液。选择254 nm波长进行检测。最后，用两种不同的数学方法对6个银黄口服液样品的指纹图谱数据进行了处理。该方法对6个银黄口服液样品显示出良好的稳定性。在银黄口服液色谱图与原料药色谱图的共有峰比较中，大部分峰能很好地匹配。
