[ HPLC法测定大花忍冬指纹图谱的研究 ]
[Study on fingerprint spectrum of Lonicera macranthoides by HPLC].
作者信息
Xin Ning, Feng Jie, Huang Lu-liang, Yin Sheng-gao
机构信息
Guangxi Traditional Chinese Medical University, Nanning 530001, China.
出版信息
Zhong Yao Cai. 2008 Aug;31(8):1138-42.
OBJECT
To establish the fingerprint spectrum of Lonicera macranthoides by HPLC.
METHODS
The column of Hypersil gold C18 (4. 6 mm x 250 mm, 5 microm) was used. The mobile phase consisted of Acetonitril-0.05% phosphoric acid with gradient elution. The column temperature was 30 degrees C, the detective wavelength was 240 nm, and the flow rate was 0.5 ml/min.
RESULTS
Fingerprint spectrum of Lonicera macranthoides was established, and 11 samples of different origin Lonicera macranthoides were detected. Ten peaks in the chromatogram were common by Similarity Evaluation System for Chromatographic Fingerprints of Traditional Chinese Medicine (Version 2004 A). There was a high similarity and each chromatographic peak was obtained with good separation and correlation according to the technical requirements of fingerprint of Chinese traditional medicine.
CONCLUSION
This method is accurate, reliable and provides a scientific basis for controlling the quality of Lonicera macranthoides.
目的
采用高效液相色谱法建立大花忍冬的指纹图谱。
方法
使用Hypersil gold C18柱（4.6 mm×250 mm，5μm）。流动相为乙腈-0.05%磷酸，采用梯度洗脱。柱温为30℃，检测波长为240 nm，流速为0.5 ml/min。
结果
建立了大花忍冬的指纹图谱，检测了11个不同产地的大花忍冬样品。通过《中药色谱指纹图谱相似度评价系统》（2004 A版），色谱图中有10个峰为共有峰。按照中药指纹图谱的技术要求，各色谱峰分离度良好，相关性高，相似度良好。
结论
该方法准确、可靠，为大花忍冬的质量控制提供了科学依据。
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