Ramasoota P, Wongwit W, Sampunachot P, Unnarat K, Ngamying M, Svenson S B
Department of Social and Environmental Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2000 Sep;31(3):493-7.
A new finding is reported of multiple mutations in the rpoB gene of 9 Mycobacterium leprae strains from leprosy patients in Thailand, who did not respond to therapy even when rifampicin, the main drug in multi-drug therapy was used. By means of sequence analysis of 9 Thai M. leprae strains, various mutations in 289 bps of the rpoB gene revealed forms of mutation never before described, such as multiple mutations (ie, mutation at two, three, six, seven, eight and nine positions in the rpoB gene), most of which were point-mutation substitutions (a few of which were silent), and some insertions. This investigation demonstrates that mutation in the rpoB gene of M. leprae strains from Thailand involves more variety than previously reported for rpoB mutation patterns in rifampicin resistance M. leprae strains.
据报道，在泰国麻风病患者的9株麻风分枝杆菌菌株的rpoB基因中发现了多个突变，这些患者即使使用了多药疗法中的主要药物利福平，也对治疗无反应。通过对9株泰国麻风分枝杆菌菌株进行序列分析，rpoB基因289个碱基对中的各种突变揭示了前所未有的突变形式，如多个突变（即rpoB基因中两个、三个、六个、七个、八个和九个位置的突变），其中大多数是点突变替代（少数为沉默突变），还有一些插入突变。这项研究表明，泰国麻风分枝杆菌菌株rpoB基因中的突变比以前报道的耐利福平麻风分枝杆菌菌株的rpoB突变模式更加多样。
