基因分析器：高通量毛细管指纹图谱数据的批量处理

GenoProfiler: batch processing of high-throughput capillary fingerprinting data.

作者信息

You Frank M, Luo Ming-Cheng, Gu Yong Qiang, Lazo Gerard R, Deal Karin, Dvorak Jan, Anderson Olin D

机构信息

Department of Plant Sciences, University of California, Davis, CA 95616, USA.

出版信息

Bioinformatics. 2007 Jan 15;23(2):240-2. doi: 10.1093/bioinformatics/btl494. Epub 2006 Oct 2.

DOI:10.1093/bioinformatics/btl494

PMID:17018534

Abstract

UNLABELLED

High-throughput content fingerprinting techniques employing capillary electrophoresis place new demands on the editing of fingerprint files for the downstream contig assembly program, FPC. A cross-platform software application, GenoProfiler, was developed for automated editing of sized fingerprinting profiles generated by the ABI Genetic Analyzers. The batch-processing module extracts the sized fragment information directly from the ABI raw trace files, or from data files exported from GeneMapper or other size calling software, removes the background noise and undesired fragments, and generates fragment size files compatible with the FPC software.

AVAILABILITY

http://wheat.pw.usda.gov/PhysicalMapping/

摘要

未标注

采用毛细管电泳的高通量内容指纹识别技术对用于下游重叠群组装程序FPC的指纹文件编辑提出了新要求。开发了一种跨平台软件应用程序GenoProfiler，用于自动编辑由ABI基因分析仪生成的大小指纹图谱。批处理模块直接从ABI原始跟踪文件或从GeneMapper或其他大小调用软件导出的数据文件中提取大小片段信息，去除背景噪声和不需要的片段，并生成与FPC软件兼容的片段大小文件。