Song Jingyuan, Lu Shanfa, Chen Zenn-Zong, Lourenco Rodrigo, Chiang Vincent L
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Xibeiwang, Haidian District, Beijing 100094, PR China.
Plant Cell Physiol. 2006 Nov;47(11):1582-9. doi: 10.1093/pcp/pcl018. Epub 2006 Oct 3.
We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium tumefaciens C58 produced transgenic calli, as confirmed by beta-glucuronidase (GUS) staining. The regeneration efficiency of independent transgenic plants was approximately 13%, as revealed by genomic Southern analysis. Some transgenic plants were produced in as little as 5 months after co-cultivation. This system may help to facilitate studies of gene functions in tree growth and development at a genome level.
我们在此报告对Nisqually-1进行农杆菌介导的遗传转化，Nisqually-1是一种毛果杨基因型，其基因组最近已被测序。建立了多个系统。来自生长旺盛的温室植物的节间茎段是最适合转化的外植体。对于最有效的系统，经β-葡萄糖醛酸酶（GUS）染色证实，约40%感染含pBI121的根癌农杆菌C58的茎段产生了转基因愈伤组织。基因组Southern分析显示，独立转基因植株的再生效率约为13%。共培养后短短5个月内就产生了一些转基因植株。该系统可能有助于在基因组水平上促进对树木生长和发育中基因功能的研究。
