Tabata Shoji, Kudo Ken-ichi, Wada-Takemura Azumi, Nishimura Shotaro, Iwamoto Hisao
Laboratory of Functional Anatomy, Department of Animal Science, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
J Vet Med Sci. 2006 Sep;68(9):953-7. doi: 10.1292/jvms.68.953.
The taste buds of bovine fungiform papillae were studied by light and electron microscopy using both histological and immunohistochemical methods. The taste buds existed in the epithelium of the apical region of the papillae. By electron microscopy, two types of taste cells, namely type I and type II cells, could be classified according to the presence of dense-cored vesicles, the cytoplasmic density and the cell shape. Type I cells were thin, had an electron-dense cytoplasm containing dense-cored vesicles, and possessed long thick apical processes in the taste pore. Type II cells were thick, had an electron-lucent cytoplasm containing many electron-lucent vesicles, rather than dense-cored vesicles, and possessed microvilli in the taste pore. Immunohistochemical staining with an antiserum against gustducin was investigated by both light and electron microscopy using the avidin-biotin complex (ABC) method. Some, but not all, of the type II cells exhibited gustducin immunoreactivity, whereas none of the type I cells showed any immunoreactivity.
采用组织学和免疫组织化学方法，通过光学显微镜和电子显微镜对牛菌状乳头的味蕾进行了研究。味蕾存在于乳头顶端区域的上皮中。通过电子显微镜观察，根据致密核心小泡的存在、细胞质密度和细胞形状，可以将两种类型的味觉细胞，即I型和II型细胞进行分类。I型细胞较薄，细胞质电子密度高，含有致密核心小泡，在味孔中有长而粗的顶端突起。II型细胞较厚，细胞质电子透明，含有许多电子透明小泡，而非致密核心小泡，在味孔中有微绒毛。使用抗味觉传导素抗血清，通过抗生物素蛋白-生物素复合物（ABC）方法，在光学显微镜和电子显微镜下进行免疫组织化学染色研究。部分（但并非全部）II型细胞呈现味觉传导素免疫反应性，而I型细胞均未显示任何免疫反应性。
