Biernat-Sudolska Małgorzata, Rojek-Zakrzewska Danuta, Lauterbach Ryszard
Department Virology, Chair of Microbiology, Medical College of Jagiellonian University, Kraków, Poland.
Acta Biochim Pol. 2006;53(3):609-11. Epub 2006 Oct 1.
We compared three methods used microbial culturing for detection of ureaplasmas in endotracheal aspirate from 500 prematurely born neonates with respiratory disturbances: BioMerieux test, PCR and microbial culturing. Ureaplasmas were detected in respiratory tracts of 79 (16%) newborns. Correlation of the results of culture with those obtained with the BioMerieux kit, culture with PCR and BioMerieux kit with PCR was 97%, 89% and 90%, respectively. Sensitivity and specificity of PCR in comparison with culture was 86% and 98%, respectively, and of the BioMerieux kit 96% and 98%. PCR can be recommended in rapid diagnostics of respiratory infections in newborns suffering from respiratory disorders. It allows the detection of ureaplasmas in case of parallel infections and identification of their species.
我们比较了三种用于检测500例患有呼吸障碍的早产新生儿气管内吸出物中脲原体的微生物培养方法：生物梅里埃检测法、聚合酶链反应（PCR）法和微生物培养法。在79名（16%）新生儿的呼吸道中检测到了脲原体。培养结果与生物梅里埃试剂盒检测结果、培养结果与PCR检测结果以及生物梅里埃试剂盒与PCR检测结果的相关性分别为97%、89%和90%。与培养法相比，PCR的敏感性和特异性分别为86%和98%，生物梅里埃试剂盒的敏感性和特异性分别为96%和98%。PCR可推荐用于患有呼吸障碍的新生儿呼吸道感染的快速诊断。它能够在并发感染的情况下检测到脲原体并鉴定其种类。
