Wischke C, Borchert H H
Department of Pharmacy, Free University of Berlin, Germany.
Pharmazie. 2006 Sep;61(9):770-4.
In the therapy of various diseases, parenterally administered protein drugs are of steadily rising importance. In order to reduce the application frequency, these proteins can be incorporated into drug delivery systems, e.g. biodegradable microparticles from poly(lactic-co-glycolic acid) (PLGA). To evaluate the characteristics of these vehicles, fluorescent labelled proteins like fluorescein isothiocyanate labelled bovine serum albumin (FITC-BSA) may be used as model drugs to allow the visualisation of the protein localisation within the microparticle and the detection of microparticles in cell cultures or tissues. However, the quantification of protein by fluorescence spectroscopy failed. In this study we focused on the mechanism of fluorescence dequenching in a multi-FITC-labelled protein and its impact on a reliable protein determination.
在各种疾病的治疗中，肠胃外给药的蛋白质药物的重要性在不断提高。为了减少给药频率，这些蛋白质可以被纳入药物递送系统，例如由聚（乳酸-乙醇酸共聚物）（PLGA）制成的可生物降解微粒。为了评估这些载体的特性，荧光标记的蛋白质如异硫氰酸荧光素标记的牛血清白蛋白（FITC-BSA）可作为模型药物，用于观察蛋白质在微粒内的定位以及检测细胞培养物或组织中的微粒。然而，通过荧光光谱法对蛋白质进行定量分析失败了。在本研究中，我们专注于多FITC标记蛋白质中荧光去猝灭的机制及其对可靠蛋白质测定的影响。
