Seok Ji Min, Kim Min Ji, Park Jin Ho, Kim Dahong, Lee Dongjin, Yeo Seon Ju, Lee Jun Hee, Lee Kangwon, Byun June-Ho, Oh Se Heang, Park Su A
Nano-Convergence Manufacturing Systems Research Division, Korea Institute of Machinery and Materials (KIMM), Daejeon, 34103, Republic of Korea.
Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, 08826, Republic of Korea.
Mater Today Bio. 2023 Jun 13;21:100685. doi: 10.1016/j.mtbio.2023.100685. eCollection 2023 Aug.
Extrusion-based bioprinting technology is widely used for tissue regeneration and reconstruction. However, the method that uses only hydrogel as the bioink base material exhibits limited biofunctional properties and needs improvement to achieve the desired tissue regeneration. In this study, we present a three-dimensionally printed bioactive microparticle-loaded scaffold for use in bone regeneration applications. The unique structure of the microparticles provided sustained release of growth factor for > 4 weeks without the use of toxic or harmful substances. Before and after printing, the optimal particle ratio in the bioink for cell viability demonstrated a survival rate of ≥ 85% over 7 days. Notably, osteogenic differentiation and mineralization-mediated by human periosteum-derived cells in scaffolds with bioactive microparticles-increased over a 2-week interval. Here, we present an alternative bioprinting strategy that uses the sustained release of bioactive microparticles to improve biofunctional properties in a manner that is acceptable for clinical bone regeneration applications.
基于挤出的生物打印技术广泛应用于组织再生与重建。然而，仅使用水凝胶作为生物墨水基础材料的方法表现出有限的生物功能特性，需要改进以实现所需的组织再生。在本研究中，我们展示了一种用于骨再生应用的三维打印的负载生物活性微粒的支架。微粒的独特结构在不使用有毒有害物质的情况下实现了生长因子超过4周的持续释放。在打印前后，生物墨水中用于细胞活力的最佳颗粒比例在7天内显示出≥85%的存活率。值得注意的是，在含有生物活性微粒的支架中，人骨膜来源细胞介导的成骨分化和矿化在2周的时间间隔内有所增加。在此，我们提出了一种替代生物打印策略，该策略利用生物活性微粒的持续释放以一种临床骨再生应用可接受的方式改善生物功能特性。
