吐温80和水溶维生素A对病毒蚀斑形成的影响。
Effect to tween 80 and aquasol A on virus plaque formation.
作者信息
Blalock J E, Tullish J, Gifford G E
出版信息
Infect Immun. 1975 Sep;12(3):490-4. doi: 10.1128/iai.12.3.490-494.1975.
The enhancement of vesicular stomatitis virus plaques on human embryonic lung cells in the presence of Tween 80 or Aquasol A was studied to determine the optimal conditions for the enhancement. Enhanced numbers and sizes of vesicular stomatitis virus plaques occurred when Aquasol A or Tween 80 was added to the cell culture 30 min before virus adsorption but not when added after adsorption. These substances did not have a direct effect on the virus and did not have an effect on virus adsorption or penetration. A few other viruses and cell systems were also studied to determine if enhancement would extend to other viruses and cell systems. The cell system seemed to be important since enhancement of vesicular stomatitis virus plating efficiency did not occur on chicken embryo cells. However, the virus was also important since vaccinia virus plating efficiency was not enhanced on the human embryonic lung cell. The greatest enhancement encountered was the increase in the plating efficiency of Friend leukemia virus on S+L- cells.
研究了在吐温80或水溶A存在的情况下，水泡性口炎病毒蚀斑在人胚肺细胞上的增强情况，以确定增强的最佳条件。当在病毒吸附前30分钟将水溶A或吐温80添加到细胞培养物中时，水泡性口炎病毒蚀斑的数量和大小会增加，但在吸附后添加则不会。这些物质对病毒没有直接影响，也不会对病毒吸附或穿透产生影响。还研究了其他一些病毒和细胞系统，以确定增强作用是否会扩展到其他病毒和细胞系统。细胞系统似乎很重要，因为在鸡胚细胞上未出现水泡性口炎病毒铺板效率的增强。然而，病毒也很重要，因为在人胚肺细胞上痘苗病毒的铺板效率没有增强。所遇到的最大增强是弗瑞德白血病病毒在S+L-细胞上的铺板效率增加。
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