Rausch P G, Canonico P G
Infect Immun. 1975 Sep;12(3):687-93. doi: 10.1128/iai.12.3.687-693.1975.
Rhesus monkey (Macaca mulatta) neutrophils were shown to contain the azurophilic granule maker enzymes myeloperoxidase and beta-glucuronidase but were deficient in the specific granule markers alkaline phosphatase (AKP) and lysozyme. Isopycnic centrifugation of leukocyte homogenates on linear sucrose gradients resulted in cosedimentation of myeloperoxidase and beta-glucuronidase with an equilibrium density of 1.18. After an intravenous inoculation of monkeys with Salmonella typhimurium AKP activity became marked, whereas that of beta-glucuronidase decreased and myeloperoxidase remained unchanged. Lysozyme was undetected throughout the course of the experiment, but was present in oil-induced peritoneal macrophages and peripheral mononuclear cells. The induced AKP exhibited partial latency and had an equilibrium density of 1.15. It is unclear, however, whether the induced AKP is associated with specific granules or cytoplasmic membranes. Hence, while these data are consistent with the presence of azurophilic granules in polymorphonuclear neutrophils from infected monkeys, the presence of specific granules in polymorphonuclear neutrophils of both uninfected and infected monkeys remains moot.
恒河猴（猕猴）的嗜中性粒细胞被证明含有嗜天青颗粒生成酶髓过氧化物酶和β-葡萄糖醛酸酶，但缺乏特异性颗粒标志物碱性磷酸酶（AKP）和溶菌酶。白细胞匀浆在线性蔗糖梯度上进行等密度离心，导致髓过氧化物酶和β-葡萄糖醛酸酶共沉降，平衡密度为1.18。给猴子静脉接种鼠伤寒沙门氏菌后，AKP活性显著增强，而β-葡萄糖醛酸酶活性降低，髓过氧化物酶活性保持不变。在整个实验过程中未检测到溶菌酶，但在油诱导的腹腔巨噬细胞和外周单核细胞中存在。诱导产生的AKP表现出部分潜伏性，平衡密度为1.15。然而，尚不清楚诱导产生的AKP是与特异性颗粒还是细胞质膜相关。因此，虽然这些数据与感染猴子的多形核嗜中性粒细胞中存在嗜天青颗粒一致，但未感染和感染猴子的多形核嗜中性粒细胞中是否存在特异性颗粒仍不确定。
