Tuve Sebastian, Wang Hongjie, Ware Carol, Liu Ying, Gaggar Anuj, Bernt Kathrin, Shayakhmetov Dmitry, Li Zongyi, Strauss Robert, Stone Daniel, Lieber André
Division of Medical Genetics, Box 357720, University of Washington, Seattle, WA 98195, USA.
J Virol. 2006 Dec;80(24):12109-20. doi: 10.1128/JVI.01370-06. Epub 2006 Oct 4.
CD46 is used by human group B adenoviruses (Ads) as a high-affinity attachment receptor. Here we show evidence that several group B Ads utilize an additional receptor for infection of human cells, which is different from CD46. We tentatively named this receptor receptor X. Competition studies with unlabeled and labeled Ads, recombinant Ad fiber knobs, and soluble CD46 and CD46 antibodies revealed three different subgroups of group B Ads, in terms of their receptor usage. Group I (Ad16, -21, -35, and -50) nearly exclusively uses CD46. Group II (Ad3, -7p, and -14) utilizes receptor X and not CD46. Group III (Ad11p) uses both CD46 and the alternative receptor X. Interaction of group II and III Ads with receptor X occurs via the fiber knob. Receptor X is an abundantly expressed glycoprotein that interacts with group II and III Ads at relatively low affinity in a Ca(2+)-dependent manner. This receptor is expressed at high levels on human mesenchymal and undifferentiated embryonic stem cells, as well as on human cancer cell lines. These findings have practical implications for stem cell and gene therapy.
人类B组腺病毒（Ads）将CD46用作高亲和力附着受体。在此我们证明，几种B组腺病毒利用一种不同于CD46的额外受体来感染人类细胞。我们暂时将此受体命名为受体X。用未标记和标记的腺病毒、重组腺病毒纤维结、可溶性CD46和CD46抗体进行的竞争研究揭示了B组腺病毒在受体使用方面的三个不同亚组。第一组（Ad16、-21、-35和-50）几乎只使用CD46。第二组（Ad3、-7p和-14）利用受体X而非CD46。第三组（Ad11p）同时使用CD46和替代受体X。第二组和第三组腺病毒与受体X的相互作用通过纤维结发生。受体X是一种大量表达的糖蛋白，它以钙依赖的方式以相对低的亲和力与第二组和第三组腺病毒相互作用。该受体在人间充质和未分化胚胎干细胞以及人类癌细胞系上高水平表达。这些发现对干细胞和基因治疗具有实际意义。
