Division of Virology, Department of Clinical Microbiology, Laboratory for Molecular Infection Medicine in Sweden, UCMR, Umeå University, SE-901 85 Umeå, Sweden.
Virology. 2010 Jun 20;402(1):20-5. doi: 10.1016/j.virol.2010.03.004. Epub 2010 Mar 27.
Hemagglutination is an established method but has not been used previously to determine the efficacy of virus binding to a specific cellular receptor. Here we have utilized CD46-expressing erythrocytes from a transgenic mouse to establish whether and to what extent the species B adenoviruses (Ads) as well as Ad37 and Ad49 of species D can interact with CD46. A number of different agglutination patterns, and hence CD46 interactions, could be observed for the different adenovirus types. In this system Ad7p, Ad11a, and Ad14 did not agglutinate mouse erythrocytes at all. Hemagglutination of CD46 expressing erythrocytes with high efficiency was observed for the previously established CD46 users Ad11p and Ad35 as well as for the less investigated Ad34. Ad50 agglutinated with moderate efficiency. Ad16, Ad21 and Ad49 gave incomplete agglutination. Ad16 was the only adenovirus that could be eluted. No specific CD46 interaction could be observed for Ad3p or for Ad37.
红细胞凝集是一种已建立的方法，但以前从未用于确定病毒与特定细胞受体结合的功效。在这里，我们利用来自转基因小鼠的表达 CD46 的红细胞来确定 B 属腺病毒（Ads）以及 D 属的 Ad37 和 Ad49 是否以及在何种程度上可以与 CD46 相互作用。对于不同的腺病毒类型，可以观察到多种不同的凝集模式，因此 CD46 的相互作用也不同。在该系统中，Ad7p、Ad11a 和 Ad14 根本不会凝集鼠红细胞。对于先前建立的 CD46 使用者 Ad11p 和 Ad35 以及研究较少的 Ad34，观察到 CD46 表达的红细胞的高效红细胞凝集。Ad50 以中等效率凝集。Ad16、Ad21 和 Ad49 给出不完全凝集。Ad16 是唯一可以洗脱的腺病毒。对于 Ad3p 或 Ad37，没有观察到特定的 CD46 相互作用。
