Hall Kathryn, Blair Zajdel Maria E, Blair G Eric
Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
Virology. 2009 Sep 30;392(2):222-9. doi: 10.1016/j.virol.2009.07.010. Epub 2009 Aug 13.
Attachment of human adenoviruses (Ads) to host cells is mediated by the interaction of the fiber protein of the capsid with specific cell-surface molecules. For one of the species B adenoviruses, Ad3, the mechanism of binding to cells remains to be defined. Several previous reports have proposed CD46, CD80 or CD86 as possible Ad3 fiber attachment molecules. In this study, CD80 and CD86 were not found to mediate Ad3 fiber binding or Ad3-EGFP transduction of cells. Low levels of Ad3-EGFP transduction of a CHO cell line expressing relatively high levels of CD46 were detected which might suggest a role for CD46 in facilitating Ad3: cell interactions, in the absence of other attachment molecules. Anti-CD46 antibodies and siRNAs had almost no effect on Ad3 fiber binding or Ad3-EGFP transduction of HeLa cells. However, treatment of A549 cells with CD46 siRNA resulted in some decrease of transduction with Ad3-EGFP.
人腺病毒（Ads）与宿主细胞的附着是由衣壳纤维蛋白与特定细胞表面分子的相互作用介导的。对于B种腺病毒之一Ad3，其与细胞结合的机制尚待确定。先前的几份报告提出CD46、CD80或CD86可能是Ad3纤维附着分子。在本研究中，未发现CD80和CD86介导Ad3纤维与细胞的结合或Ad3-EGFP对细胞的转导。在表达相对高水平CD46的CHO细胞系中检测到低水平的Ad3-EGFP转导，这可能表明在没有其他附着分子的情况下，CD46在促进Ad3与细胞相互作用中发挥作用。抗CD46抗体和小干扰RNA（siRNAs）对HeLa细胞的Ad3纤维结合或Ad3-EGFP转导几乎没有影响。然而，用CD46 siRNA处理A549细胞导致Ad3-EGFP转导有所下降。
