Fuchsman Clara A, Rocap Gabrielle
School of Oceanography, Box 357940, University of Washington, Seattle, WA 98195, USA.
Appl Environ Microbiol. 2006 Oct;72(10):6841-4. doi: 10.1128/AEM.00429-06.
The genome sequences of Rhodopirellula baltica, formerly Pirellula sp. strain 1, Blastopirellula marina, Gemmata obscuriglobus, and Kuenenia stuttgartiensis were used in a series of pairwise reciprocal best-hit analyses to evaluate the contested evolutionary position of Planctomycetes. Contrary to previous reports which suggested that R. baltica had a high percentage of genes with closest matches to Archaea and Eukarya, we show here that these Planctomycetes do not share an unusually large number of genes with the Archaea or Eukarya, compared with other Bacteria. Thus, best-hit analyses may assign phylogenetic affinities incorrectly if close relatives are absent from the sequence database.
波罗的海玫瑰杆菌(以前称为皮氏菌属菌株1)、海芽殖杆菌、隐匿球状体菌和斯图加特库内尼亚菌的基因组序列被用于一系列两两相互最佳匹配分析,以评估浮霉菌门有争议的进化位置。与之前表明波罗的海玫瑰杆菌有高比例基因与古菌和真核生物最匹配的报告相反,我们在此表明,与其他细菌相比,这些浮霉菌与古菌或真核生物并不共享异常大量的基因。因此,如果序列数据库中没有近亲,最佳匹配分析可能会错误地确定系统发育亲缘关系。