Ravid A, Tamir R, Liberman U A, Rotem C, Pick A I, Novogrodsky A, Koren R
Rogoff Medical Research Institute, Beilinson Medical Center, Petah-Tikva, Israel.
J Allergy Clin Immunol. 1990 Dec;86(6 Pt 1):881-5. doi: 10.1016/s0091-6749(05)80150-2.
The inhibitory effect of prostaglandin E2, histamine, isobutylmethylxanthine, and 1,25-dihydroxyvitamin D3 (1,25-[OH]2D3) on the mitogenic stimulation of peripheral blood lymphocytes from normal and atopic subjects was studied. We found that lymphocytes from atopic patients were less susceptible to inhibition by the three agents that elevate intracellular cyclic adenosine monophosphate (cAMP) concentrations and by the active metabolite of vitamin D (inhibition of 27%, 14%, 12%, and 36% for the atopic patients as compared with 40%, 20%, 22%, and 46% for the normal donors, by the four agents, respectively; p less than 0.02). The inhibitory effect of the cAMP-elevating agents was potentiated by the addition of 1,25-(OH)2D3 to the lymphocyte cultures. The potentiation was more pronounced on lymphocytes from the atopic donors, increasing their responsiveness to levels comparable to levels of lymphocytes from normal donors. The synthetic corticosteroid, dexamethasone, had a similar potentiating effect on the inhibitory action of prostaglandin E2. In view of the beneficial action of beta-agonists, phosphodiesterase inhibitors, and corticosteroids in the treatment of allergy, the potentiating effect of 1,25-(OH)2D3 on the action of cAMP-elevating agents may be of therapeutic interest.
研究了前列腺素E2、组胺、异丁基甲基黄嘌呤和1,25 - 二羟基维生素D3（1,25-[OH]2D3）对正常和特应性受试者外周血淋巴细胞有丝分裂刺激的抑制作用。我们发现，特应性患者的淋巴细胞对三种能提高细胞内环磷酸腺苷（cAMP）浓度的药物以及维生素D的活性代谢物的抑制作用较不敏感（四种药物对特应性患者的抑制率分别为27%、14%、12%和36%，而对正常供体分别为40%、20%、22%和46%；p<0.02）。在淋巴细胞培养物中添加1,25-(OH)2D3可增强提高cAMP的药物的抑制作用。这种增强作用在特应性供体的淋巴细胞上更为明显，使其反应性提高到与正常供体淋巴细胞相当的水平。合成皮质类固醇地塞米松对前列腺素E2的抑制作用有类似的增强作用。鉴于β-激动剂、磷酸二酯酶抑制剂和皮质类固醇在治疗过敏方面的有益作用，1,25-(OH)2D3对提高cAMP药物作用的增强作用可能具有治疗意义。
