Bogomolova N N, Chaplygina N M, Ataullakhanov A I, Burgasova M P, Nevraeva E G, Filimonova R G
Mol Gen Mikrobiol Virusol. 1990 Oct(10):26-7.
The presence of the measles (rubeola) virus genome was searched for in the lymphocytes from the peripheral blood of patients suffering from the acute (16 persons) or chronic (164 persons) glomerulonephritis. Dot hybridization technique with the plasmid borne probes to the measles viral genes NP, P and H have been used for the search. The measles viral genome has been detected in 58% of lymphocytes from the patients with the chronic glomerulonephritis and in 50% of lymphocytes from the patients suffering from the acute form of the disease. The genome was not found in the material from the control group including donors and traumatology ward patients. 25 samples of lymphocytes from the patients with the chronic glomerulonephritis contained the RNA that was not hybridizable with the viral genes probes by dot hybridization technique, thus containing no genes homologous to parotitis viral genes. The average titer of anti-measles antibodies in the serum from patients with chronic glomerulonephritis the lymphocytes of which contained the measles viral genome was 1:304, while it was 1:154 for patients with the negative probes. The average anti-measles antibodies titers are the same (1:166 and 1:142) for analogical groups of patients with acute form of disease.
在患有急性肾小球肾炎（16例）或慢性肾小球肾炎（164例）患者的外周血淋巴细胞中搜索麻疹（风疹）病毒基因组。采用针对麻疹病毒基因NP、P和H的质粒载体探针的点杂交技术进行搜索。在慢性肾小球肾炎患者的58%淋巴细胞以及急性肾小球肾炎患者的50%淋巴细胞中检测到麻疹病毒基因组。在包括献血者和创伤科病房患者的对照组样本中未发现该基因组。25例慢性肾小球肾炎患者的淋巴细胞样本经点杂交技术检测，所含RNA不能与病毒基因探针杂交，因此不含与腮腺炎病毒基因同源的基因。淋巴细胞中含有麻疹病毒基因组的慢性肾小球肾炎患者血清中抗麻疹抗体的平均滴度为1:304，而探针检测为阴性的患者该抗体平均滴度为1:154。急性肾小球肾炎患者的类似分组中，抗麻疹抗体平均滴度相同（分别为1:166和1:142）。
