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Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents.

作者信息

Osswald M, Greuer B, Brimacombe R

机构信息

Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin-Dahlem, FRG.

出版信息

Nucleic Acids Res. 1990 Dec 11;18(23):6755-60. doi: 10.1093/nar/18.23.6755.

DOI:10.1093/nar/18.23.6755
PMID:1702198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC332727/
Abstract

50S ribosomal subunits were reacted with bis-(2-chloroethyl)methylamine, 2-iminothiolane or methyl p-azidophenyl acetimidate, and RNA-protein cross-link sites on the RNA were localised using our published procedures. The degree of precision with which these sites could be determined was variable, depending on the particular protein or RNA region concerned. The following positions in the 23S RNA were identified as encompassing the individual cross-link sites (numbered from the 5'-end, with asterisks denoting sites previously reported): L1, 1864-67, 1876-78, 2119-33, 2163-72*, L2, 1819-20*; L3, 2832-34; L4, 320-25*; 613-17*; L5, 2307; L6, 2473-81*; L9, 1484-91; L11, 1060-62; L13, 547-50; L14, 1993-2002; L17, 1260-95; L18, 2307-20; L19, 1741-58; L21, 544-48*; 1198-1248; L23, 63-65, 137-41*; L24, 99-107*; L27, 2272-83, 2320-23*; 2332-37*; L28, 195-242, 368-424; L29, 101-02*; L30, 931-38; L32, 2878-90; L33, 2422-24. Cross-links to 5S RNA were observed with L5 (positions 34-41), and L18 (precise site not localised).

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf85/332727/b5f3afabf1bb/nar00207-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf85/332727/b5f3afabf1bb/nar00207-0020-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf85/332727/b5f3afabf1bb/nar00207-0020-a.jpg

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1
Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents.
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2
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3
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本文引用的文献

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J Mol Biol. 1984 Nov 5;179(3):431-52. doi: 10.1016/0022-2836(84)90074-3.
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The binding site for ribosomal protein complex L8 within 23 s ribosomal RNA of Escherichia coli.核糖体蛋白复合物L8在大肠杆菌23 s核糖体RNA中的结合位点。
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The localization of multiple sites on 16S RNA which are cross-linked to proteins S7 and S8 in Escherichia coli 30S ribosomal subunits by treatment with 2-iminothiolane.
紫外线B对玉米核糖体中核糖体蛋白与RNA的交联作用及其对翻译的影响。
Plant Physiol. 2004 Oct;136(2):3319-32. doi: 10.1104/pp.104.047043. Epub 2004 Oct 1.
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J Biol Chem. 2003 Jul 25;278(30):28237-45. doi: 10.1074/jbc.M302651200. Epub 2003 May 8.
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RNA. 2002 Mar;8(3):279-89. doi: 10.1017/s135583820202407x.
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Covariance of complementary rRNA loop nucleotides does not necessarily represent functional pseudoknot formation in vivo.互补rRNA环核苷酸的协方差不一定代表体内功能性假结的形成。
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通过用2-亚氨基硫杂环戊烷处理，对大肠杆菌30S核糖体亚基中与蛋白质S7和S8交联的16S RNA上多个位点的定位。
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Nucleic Acids Res. 1981 Sep 11;9(17):4285-302. doi: 10.1093/nar/9.17.4285.
6
Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.来自大肠杆菌的23S核糖体RNA基因的完整核苷酸序列。
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Structure and function of ribosomal RNA.核糖体RNA的结构与功能。
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8
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J Mol Biol. 1988 Mar 5;200(1):65-87. doi: 10.1016/0022-2836(88)90334-8.
9
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10
Intra-RNA and RNA-protein cross-linking techniques in Escherichia coli ribosomes.大肠杆菌核糖体中的RNA内部及RNA-蛋白质交联技术
Methods Enzymol. 1988;164:287-309. doi: 10.1016/s0076-6879(88)64050-x.