Karioti A, Vrahimi-Hadjilouca T, Droushiotis D, Rancic A, Hadjipavlou-Litina D, Skaltsa H
Department of Pharmacognosy and Chemistry of Natural Products, School of Pharmacy, University of Athens, Athens, Greece.
Planta Med. 2006 Nov;72(14):1330-4. doi: 10.1055/s-2006-947255. Epub 2006 Oct 4.
The volatile composition of Origanum dubium in two different maturation stages has been studied. The essential oils were obtained by hydrodistillation in a modified Clevenger-type apparatus, and their analyses were performed by GC and GC-MS. Identification of the components was made by comparison of mass spectra and retention indices with literature records and by co-chromatography with authentic compounds. Carvacrol was shown to be the main constituent. The essential oils were evaluated for antimicrobial activity and proved to be active against all tested microorganisms. Furthermore, their potential antioxidant activity was investigated and found to be significant in scavenging O2-. The samples were further evaluated for inhibition of soybean lipoxygenase LOX and showed high inhibitory activity.
研究了两个不同成熟阶段的牛至（Origanum dubium）的挥发性成分。通过在改良的克莱文杰型装置中进行水蒸馏获得精油，并通过气相色谱（GC）和气相色谱-质谱联用（GC-MS）对其进行分析。通过将质谱和保留指数与文献记录进行比较以及与标准化合物进行共色谱分析来鉴定成分。结果表明，香芹酚是主要成分。对精油的抗菌活性进行了评估，结果证明其对所有测试微生物均有活性。此外，还研究了它们潜在的抗氧化活性，发现其在清除超氧阴离子（O2-）方面具有显著作用。对样品进一步评估了其对大豆脂氧合酶（LOX）的抑制作用，结果显示具有高抑制活性。
