来自香蚤草的两种酚类化合物的分离及抗菌活性

Isolation and antimicrobial activity of two phenolic compounds from Pulicaria odora L.

作者信息

Ezoubeiri A, Gadhi C A, Fdil N, Benharref A, Jana M, Vanhaelen M

机构信息

Laboratory of Phytochemistry and Medicinal Plants, Department of Biology, Faculty of Sciences, Semlalia, B.P. 2390, Marrakesh 40 000, Morocco.

出版信息

J Ethnopharmacol. 2005 Jun 3;99(2):287-92. doi: 10.1016/j.jep.2005.02.015. Epub 2005 Apr 7.

DOI:10.1016/j.jep.2005.02.015

PMID:15894140

Abstract

The essential oil of Pulicaria odora, a Moroccan medicinal plant; was analyzed by GC-MS, and subjected to column chromatography on silica gel. Two major constituents were isolated and identified as 2-isopropyl-4-methylphenol (1) and isobutyric acid 2-isopropyl-4-methylphenylester (2), by analysis of spectroscopic data (MS, 1H NMR, 13C NMR, DEPT, COSY, HMQC and HMBC experiments). The isolated compounds are reported for the first time from Pulicaria genus. The essential oil and its major constituents (compounds 1 and 2) were examined for antibacterial and antifungal activity in vitro using the diffusion and dilution methods. Results showed that the essential oil and the 2-isopropyl-4-methylphenol (1) exhibited a very significant antibacterial and antifungal activity, while the isobutyric acid 2-isopropyl-4-methylphenylester (2) was inactive for all tested strains.

摘要

摩洛哥药用植物香蚤草的精油通过气相色谱 - 质谱联用（GC-MS）进行分析，并在硅胶上进行柱色谱分离。通过光谱数据（质谱、¹H核磁共振、¹³C核磁共振、DEPT、COSY、HMQC和HMBC实验）分析，分离并鉴定出两种主要成分，分别为2 - 异丙基 - 4 - 甲基苯酚（1）和2 - 异丙基 - 4 - 甲基苯基异丁酸酯（2）。从蚤草属植物中首次报道了分离得到的这些化合物。采用扩散法和稀释法对精油及其主要成分（化合物1和2）进行了体外抗菌和抗真菌活性研究。结果表明，精油和2 - 异丙基 - 4 - 甲基苯酚（1）表现出非常显著的抗菌和抗真菌活性，而2 - 异丙基 - 4 - 甲基苯基异丁酸酯（2）对所有测试菌株均无活性。