Bowen J G, Robins R A, Baldwin R W
Int J Cancer. 1975 Apr 15;15(4):640-50. doi: 10.1002/ijc.2910150413.
Sera from rats bearing a progressively growing transplanted aminoazo-dye-induced hepatoma (hepatoma D23) have been examined for the presence of hepatoma D23-specfic antigen, antibody and immune complexes throughout the course of tumour growth. The levels of these factors have been correlated with the in vitro blocking and inhibition of cytotoxic lymph-node cells from immunized animals for cultured tumour cells. Sera from animals bearing small tumours (7-14 days after tumour implantation contain free tumour-specific antigen whilst immune complexes could not be detected. Although these sera were neither blocking nor inhibitory under the normal conditions of the test, when concentrated two and fourfold, inhibition but not blocking of lymph-node cell cytotoxicity could be detected. In comparison sera from animals bearing large tumours (24-28 days) blocked but did no inhibit in vitro lymph-node cell cytotoxicity and this correlates with the presence of tumour-specific immune complexes in antibody excess. Animals with intermediate-sized tumours had high levels of both blocking and inbibitory activity in the serum, these effects becoming apparent when neither free antibody nor free antigen could be detected. The relevance of these findings to the mechanism by which a growing tumour may escape specific cellular immune destruction is discussed.
对患有逐渐生长的移植性氨基偶氮染料诱导肝癌（肝癌D23）的大鼠血清，在肿瘤生长全过程中检测了肝癌D23特异性抗原、抗体及免疫复合物的存在情况。这些因子的水平与免疫动物的细胞毒性淋巴结细胞对培养肿瘤细胞的体外阻断及抑制作用相关。荷小肿瘤动物（肿瘤植入后7 - 14天）的血清含有游离肿瘤特异性抗原，而未检测到免疫复合物。虽然在正常检测条件下这些血清既无阻断作用也无抑制作用，但浓缩两倍和四倍后，可检测到对淋巴结细胞细胞毒性的抑制作用而非阻断作用。相比之下，荷大肿瘤动物（24 - 28天）的血清可阻断但不抑制体外淋巴结细胞的细胞毒性，这与抗体过量时肿瘤特异性免疫复合物的存在相关。肿瘤大小中等的动物血清中同时具有高水平的阻断和抑制活性，当未检测到游离抗体和游离抗原时，这些作用变得明显。讨论了这些发现与生长中的肿瘤可能逃避特异性细胞免疫破坏机制的相关性。
