Smitsaart E N, Saiz J C, Yedloutschnig R J, Morgan D O
Departmento de Virologia, INTA-Castelar, Argentina.
Vet Immunol Immunopathol. 1990 Nov;26(3):251-65. doi: 10.1016/0165-2427(90)90095-a.
Foot-and-mouth disease (FMD) prevention and control programs are dependent upon rapid, reliable diagnostic procedures. The widely used FMD diagnostic complement fixation (CF) procedures require a specific antiserum for each of the seven FMDV serotypes making the tests both cumbersome and difficult to standardize. An FMD diagnostic, monoclonal antibody based inhibition-ELISA procedure was developed. The test uses a single monoclonal antibody (MAb) that reacts with all European and South American FMDV isolates examined. The procedure detects a highly conserved epitope on the 12S protein subunit of FMDV which appears to be common to all FMDV's with the exception of the South African Territories 2 serotype. The results indicate that the sensitivity of this test is greater than CF and approaches that of virus isolation.
口蹄疫（FMD）防控计划依赖于快速、可靠的诊断程序。广泛使用的口蹄疫诊断补体结合（CF）程序针对七种口蹄疫病毒（FMDV）血清型中的每一种都需要一种特定的抗血清，这使得测试既繁琐又难以标准化。一种基于单克隆抗体的口蹄疫诊断抑制酶联免疫吸附测定（ELISA）程序被开发出来。该测试使用一种单一的单克隆抗体（MAb），它能与所有检测的欧洲和南美FMDV分离株发生反应。该程序检测FMDV 12S蛋白亚基上一个高度保守的表位，除南非领土2型血清型外，该表位似乎在所有FMDV中都很常见。结果表明，该测试的灵敏度高于CF，接近病毒分离的灵敏度。
