Pan Sheng, Wang Yan, Quinn Joseph F, Peskind Elaine R, Waichunas Dana, Wimberger Jake T, Jin Jinghua, Li Jane G, Zhu David, Pan Catherine, Zhang Jing
Department of Pathology, University of Washington, Seattle, Washington 98104, USA.
J Proteome Res. 2006 Oct;5(10):2769-79. doi: 10.1021/pr060251s.
Biomarkers are pressingly needed to assist with the clinical diagnosis of neurodegenerative diseases and/or the monitoring of disease progression. Glycoproteins are enriched in bodily fluids such as human cerebrospinal fluid (CSF), an ideal source for discovering biomarkers due to its proximity to the central nervous system (CNS), and consequently can serve as diagnostic and/or therapeutic markers for CNS diseases. We report here an in-depth identification of glycoproteins in human CSF using a complementary proteomic approach which integrated hydrazide chemistry and lectin affinity column for glycoprotein enrichment, followed by multidimensional chromatography separation and tandem mass spectrometric analysis. Using stringent criteria, a total of 216 glycoproteins, including many low-abundance proteins, was identified with high confidence. Approximately one-third of these proteins was already known to be relevant to the CNS structurally or functionally. This investigation, for the first time, not only categorized many glycoproteins in human CSF but also expanded the existing overall CSF protein database.
迫切需要生物标志物来辅助神经退行性疾病的临床诊断和/或监测疾病进展。糖蛋白在诸如人脑脊液（CSF）等体液中含量丰富，由于其与中枢神经系统（CNS）接近，是发现生物标志物的理想来源，因此可作为中枢神经系统疾病的诊断和/或治疗标志物。我们在此报告了一种使用互补蛋白质组学方法对人脑脊液中的糖蛋白进行深入鉴定的方法，该方法整合了酰肼化学和凝集素亲和柱用于糖蛋白富集，随后进行多维色谱分离和串联质谱分析。使用严格的标准，共高可信度地鉴定出216种糖蛋白，包括许多低丰度蛋白。这些蛋白中约有三分之一在结构或功能上已被认为与中枢神经系统相关。这项研究首次不仅对人脑脊液中的许多糖蛋白进行了分类，还扩展了现有的整体脑脊液蛋白质数据库。
