Department of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX 79409, USA.
Molecules. 2021 Mar 19;26(6):1712. doi: 10.3390/molecules26061712.
Cerebrospinal fluid (CSF) contains valuable biological and neurological information. However, its glycomics analysis is hampered due to the low amount of protein in the biofluid, as has been demonstrated by other glycomics studies using a substantial amount of CSF. In this work, we investigated different -glycan sample preparation approaches to develop a more sensitive method. These methods, one with an increased amount of buffer solution during the -glycan release step with a lower amount of sample volume and the other with Filter-Aided -Glycan Separation (FANGS), were compared with recent work to demonstrate their effectiveness. It was demonstrated that an increased amount of buffer solution showed higher intensity in comparison to the previously published method and FANGS. This suggested that digestion efficiency during the -glycan release step was not in an optimal condition from the previously published method, and that there is a substantial loss of sample with FANGS when preparing -glycans from CSF.
脑脊液（CSF）中含有有价值的生物学和神经学信息。然而，由于生物体液中蛋白质的含量较低，其他使用大量 CSF 的糖组学研究已经证明了这一点，因此对其进行糖组学分析受到了阻碍。在这项工作中，我们研究了不同的 -聚糖样品制备方法，以开发一种更灵敏的方法。这些方法，一种是在 -聚糖释放步骤中增加缓冲液的量，同时减少样品的体积，另一种是使用 Filter-Aided -Glycan Separation（FANGS），与最近的工作进行了比较，以证明它们的有效性。结果表明，与之前发表的方法和 FANGS 相比，增加缓冲液的量显示出更高的强度。这表明在 -聚糖释放步骤中，消化效率不是最佳的，而 FANGS 在从 CSF 中制备 -聚糖时会有大量的样品损失。
