正常人类1号染色体对柯斯顿鼠肉瘤病毒转化的NIH/3T3细胞系的各种表型具有抑制活性。
Normal human chromosome 1 carries suppressor activity for various phenotypes of a Kirsten murine sarcoma virus-transformed NIH/3T3 cell line.
作者信息
Yamada H, Horikawa I, Hashiba H, Oshimura M
机构信息
Department of Molecular and Cell Genetics, School of Life Sciences, Faculty of Medicine, Tottori University, Yonago.
出版信息
Jpn J Cancer Res. 1990 Nov;81(11):1095-100. doi: 10.1111/j.1349-7006.1990.tb02519.x.
In order to identify chromosomes that carry putative tumor-suppressor genes for the various phenotypes of Kirsten sarcoma virus-transformed NIH/3T3 (DT) cells, we performed microcell-mediated chromosome transfer into DT cells. We first isolated mouse A9 clones, containing a single human chromosome 1, 11 or 12 tagged with pSV2-neo plasmid DNA. Then, chromosome 1, 11 or 12 was transferred from the A9 clones into DT cells by microcell fusion. The growth rate, colony-forming ability in soft agar and tumorigenicity of the DT cells were controlled by chromosome 1, but not by chromosome 11 or 12, indicating that normal human chromosome 1 carries a putative tumor-suppressor gene(s) that affects various transformed phenotypes of DT cells.
为了鉴定携带针对 Kirsten 肉瘤病毒转化的 NIH/3T3（DT）细胞各种表型的假定肿瘤抑制基因的染色体，我们进行了微细胞介导的染色体转移到 DT 细胞中。我们首先分离出含有一条用 pSV2-neo 质粒 DNA 标记的人 1 号、11 号或 12 号染色体的小鼠 A9 克隆。然后，通过微细胞融合将 1 号、11 号或 12 号染色体从 A9 克隆转移到 DT 细胞中。DT 细胞的生长速率、软琼脂中的集落形成能力和致瘤性受 1 号染色体控制，但不受 11 号或 12 号染色体控制，这表明正常的人 1 号染色体携带影响 DT 细胞各种转化表型的假定肿瘤抑制基因。
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