Tsuchida N, Ryder T, Ohtsubo E
Science. 1982 Sep 3;217(4563):937-9. doi: 10.1126/science.6287573.
The transforming protein of Kirsten murine sarcoma virus (Ki-MuSV) is a virally encoded 21-kilodalton protein called p21 kis. The sequences encoding p21 kis were genetically localized to a 1.3-kilobase segment near the 5' end of the viral genome by assaying the capacity of a series of defined deletion mutants of molecularly cloned Ki-MuSV DNA to induce focal transformation of mouse cells. Nucleotide sequencing of a portion of this region has led to the identification of an open reading frame of 567 nucleotides coding for p21 kis protein.
克尔斯滕鼠肉瘤病毒（Ki-MuSV）的转化蛋白是一种病毒编码的21千道尔顿蛋白，称为p21kis。通过检测一系列分子克隆的Ki-MuSV DNA的特定缺失突变体诱导小鼠细胞灶性转化的能力，将编码p21kis的序列基因定位到病毒基因组5'端附近的一个1.3千碱基片段上。对该区域一部分的核苷酸测序已导致鉴定出一个567个核苷酸的开放阅读框，其编码p21kis蛋白。
