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The first detection of Neospora caninum DNA in the colostrum of infected cows.

作者信息

Moskwa Bozena, Pastusiak Katarzyna, Bien Justyna, Cabaj Wladyslaw

机构信息

Witold Stefański Institute of Parasitology of the Polish Academy of Sciences, 51/55 Twarda Street, 00-818, Warsaw, Poland.

出版信息

Parasitol Res. 2007 Feb;100(3):633-6. doi: 10.1007/s00436-006-0288-7. Epub 2006 Oct 6.

DOI:10.1007/s00436-006-0288-7
PMID:17024360
Abstract

Limited data is available on the vertical transmission of Neospora caninum via the colostrum. The results of our previous research revealed the presence of N. caninum DNA in the milk of seropositive cows. The aim of the present work is to demonstrate parasite DNA in colostrum samples. A polymerase chain reaction using Np21 and Np6 primers was applied to DNA isolated from the colostrum sediment in order to amplify the corresponding genomic Nc-5 region. The expected 328-bp product was obtained in colostrum samples collected both on the calving day and the day after. This is the first detection of N. caninum DNA in the colostrum of seropositive cows, and these findings implicate the possibility of N. caninum transmission through the colostrum.

摘要

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