Eeltink Sebastiaan, Svec Frantisek, Fréchet Jean M J
Department of Chemistry, University of California, Berkeley, CA 94720-1460, USA.
Electrophoresis. 2006 Nov;27(21):4249-56. doi: 10.1002/elps.200600259.
Open-tubular columns for CEC separations having inner-wall coated with a thin layer of porous monolithic polymer have been studied. A two-step process including (i) UV-initiated polymerization leading to a layer of porous poly(butyl methacrylate-co-ethylene dimethacrylate), and (ii) UV-initiated grafting of ionizable monomers appear to be well suited for the preparation of these columns. The thickness of the porous polymer layer is controlled by the percentage of monomers in the polymerization mixture and/or length of the irradiation time. The layer thickness significantly affects retention, efficiency, and resolution in open-tubular CEC. Under optimized conditions, column efficiencies up to 400,000 plates/m can be achieved. Use of higher temperature and application of pressure enables a significant acceleration of the open-tubular CEC separations.
对内壁涂覆有薄层多孔整体聚合物的用于毛细管电色谱分离的开管柱进行了研究。一个两步法包括：（i）紫外线引发聚合反应，形成一层多孔聚（甲基丙烯酸丁酯 - 共 - 二甲基丙烯酸乙烯酯），以及（ii）紫外线引发可电离单体的接枝，似乎非常适合制备这些柱子。多孔聚合物层的厚度由聚合混合物中单体的百分比和/或照射时间的长度控制。该层厚度显著影响开管毛细管电色谱中的保留、效率和分离度。在优化条件下，可实现高达400,000塔板/米的柱效。使用更高的温度和施加压力能够显著加速开管毛细管电色谱分离。
