State Key Laboratory of Chemo/Biosensing & Chemometrics, College of Chemistry & Chemical Engineering, Hunan University, Changsha, P. R. China.
Electrophoresis. 2012 Jul;33(13):2005-11. doi: 10.1002/elps.201100714.
One-pot synthesis of porous polymer monolith decorated with N-methylimidazolium in a capillary was described. The polymer matrix was synthesized by in situ copolymerization and quaterization of 3-chloro-2-hydroxylpropyl methacrylate (CHPMA), ethylene dimethacrylate (EDMA), and N-methylimidazole (N-MIz). The influencing factors including amount of cross-linkers, composition of porogenic solvents, and polymerization temperature on the formation of the monolithic column were investigated. The monolithic column exhibited high column efficiency for thiourea, up to 135 000 plates per meter, and phenylmethanol, up to 102 000 plates per meter. Different types of compounds including alkylbenzenes, phenols, and inorganic anions were successfully baseline separated by capillary electrochromatography (CEC). The separation of theses analytes on the column indicated typical reversed-phase and anion-exchange chromatographic retention mechanism.
描述了一种在毛细管内用 N-甲基咪唑修饰的多孔聚合物整体的一锅合成方法。聚合物基质是通过 3-氯-2-羟丙基甲基丙烯酸酯（CHPMA）、二乙二醇二甲基丙烯酸酯（EDMA）和 N-甲基咪唑（N-MIz）的原位共聚和季铵化反应合成的。考察了交联剂用量、致孔剂组成和聚合温度等因素对整体柱形成的影响。该整体柱对硫脲的柱效高达 135000 板/米，对苯甲醇的柱效高达 102000 板/米。通过毛细管电色谱（CEC）成功地基线分离了不同类型的化合物，包括烷基苯、酚类和无机阴离子。这些分析物在该柱上的分离表明了典型的反相和阴离子交换色谱保留机制。
