Zaidi Shabi Abbas, Cheong Won Jo
Department of Chemistry and Nano Fine Center, Inha University, Incheon, South Korea.
Electrophoresis. 2009 May;30(9):1603-7. doi: 10.1002/elps.200800541.
Long open tubular S-ketoprofen molecule imprinted polymer capillary columns (1, 2, and 3 m) with the number of theoretical plates (N) over a million for both chrial and non-chiral CEC separation have been prepared. Chiral separation of racemic ketoprofen and non-chiral separation of other profen drugs have been achieved with excellent efficiency and good selectivity after optimization of the chromatographic conditions. The N value per column was not proportional to the effective column length although it was increased with the column length. This study has shown the outstanding prospect of the strategy to form a porous and rugged open tubular layer in a moderately long (1 m) capillary column in molecule imprinted polymer-CEC studies.
制备了长开管型S-酮洛芬分子印迹聚合物毛细管柱（1米、2米和3米），用于手性和非手性毛细管电色谱分离时理论塔板数（N）超过百万。通过优化色谱条件，实现了外消旋酮洛芬的手性分离以及其他洛芬类药物的非手性分离，分离效率高且选择性好。尽管柱效随柱长增加，但每根柱子的N值与有效柱长不成正比。本研究表明，在分子印迹聚合物-毛细管电色谱研究中，在中等长度（1米）的毛细管柱中形成多孔且坚固的开管层这一策略具有广阔前景。
