Molnar A, Kulcsár P, Prestwich G D
Department of Microbiology, Semmelweis University Medical School, Budapest, Hungary.
Biotechniques. 1990 Nov;9(5):534, 536.
The destaining of Coomassie Brilliant Blue-stained polyacrylamide gels usually is time consuming and requires large volumes of destaining solution. Removal of the Coomassie blue stain from the gel can be accomplished efficiently and economically by liquid-liquid extraction using a biphasic mixture of 1-butanol and 5% aqueous acetic acid.
考马斯亮蓝染色的聚丙烯酰胺凝胶脱色通常很耗时，且需要大量脱色液。通过使用正丁醇和5%乙酸水溶液的双相混合物进行液 - 液萃取，可以高效且经济地从凝胶上去除考马斯蓝染料。
