Kurien Biji T, Dorri Yaser
Department of Veterans Affairs, University of Oklahoma Health Sciences Center, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Methods Mol Biol. 2012;869:607-16. doi: 10.1007/978-1-61779-821-4_55.
Toxic reagents are employed to destain Coomassie Brilliant Blue (CBB) stained gels. We tested the efficacy of various paper adsorbents in adsorbing CBB released from gels during destaining. Kimwipes were the most efficient, followed by Teri towels, multifold towels, and Whatman (numbers 1 and 3) filter papers. Three Kimwipes added during destaining of a CBB-stained mini-gel helped adsorb the released dye. Thus, stain removal with Kimwipes helps reduce destain use and organic waste accumulation, enables recycling of nonradioactive destaining solution, and is 7.5-fold cheaper than an available method for CBB disposal. Next, we used Kimwipes to deplete the dye from a used CBB staining solution awaiting proper disposal by our Institutional Safety Office. Seventy-five Kimwipes successfully helped remove the dye from a 0.05% CBB staining solution in 5 to 10 min. The blue-colored Kimwipes did not release the stain even when squeezed dry after incubation in various salts, water, or acid solutions for five weeks. The CBB removed thus can be simply disposed as solid waste and will not leach out from solid landfills. Kimwipes, thus, enables CBB disposal in an environmentally friendly manner and allows recycling of destaining solution.
使用有毒试剂对考马斯亮蓝（CBB）染色的凝胶进行脱色。我们测试了各种纸质吸附剂在吸附脱色过程中从凝胶中释放出的CBB方面的效果。Kimwipes擦拭布效率最高，其次是Teri毛巾、多层毛巾和Whatman（1号和3号）滤纸。在对CBB染色的迷你凝胶进行脱色时添加三张Kimwipes擦拭布有助于吸附释放出的染料。因此，用Kimwipes擦拭布去除污渍有助于减少脱色剂的使用和有机废物的积累，能够使非放射性脱色溶液循环利用，并且比现有的CBB处理方法便宜7.5倍。接下来，我们用Kimwipes擦拭布从等待我们机构安全办公室妥善处理的用过的CBB染色溶液中去除染料。七十五张Kimwipes擦拭布在5到10分钟内成功地帮助从0.05%的CBB染色溶液中去除了染料。即使在各种盐溶液、水溶液或酸溶液中孵育五周后拧干，蓝色的Kimwipes擦拭布也不会释放出污渍。这样去除的CBB可以简单地作为固体废物处理，并且不会从固体垃圾填埋场中渗出。因此，Kimwipes擦拭布能够以环保的方式处理CBB，并允许脱色溶液循环利用。
