May P C, Lampert-Etchells M, Johnson S A, Poirier J, Masters J N, Finch C E
Andrus Gerontology Center, University of Southern California, Los Angeles 90089.
Neuron. 1990 Dec;5(6):831-9. doi: 10.1016/0896-6273(90)90342-d.
A hippocampal poly(A) RNA, pADHC-9, was cloned by differential screening of a human hippocampal cDNA library. By RNA blot analysis, pADHC-9 was elevated 2-fold in Alzheimer's disease hippocampus. In situ analyses identified pADHC-9 expression in pyramidal and non-pyramidal cells of the hippocampus and entorhinal cortex. Nucleotide sequence analysis identified pADHC-9 as a potential human homolog of rat sulfated glycoprotein 2 (SGP-2). SGP-2 expression increased in rat hippocampus following experimental lesions that mimic intrinsic neuronal loss and/or deafferentation. The function of pADHC-9 in brain has not been defined, but in serum, a similar protein inhibits complement-dependent cytolysis. Increased expression of pADHC-9 in Alzheimer's disease hippocampus may be a compensatory response mounted to retard a complement-driven neurodegenerative cascade.
通过对人海马cDNA文库进行差异筛选，克隆出一种海马多聚腺苷酸RNA，即pADHC - 9。通过RNA印迹分析，发现pADHC - 9在阿尔茨海默病海马中升高了2倍。原位分析确定pADHC - 9在海马和内嗅皮质的锥体细胞和非锥体细胞中表达。核苷酸序列分析确定pADHC - 9为大鼠硫酸化糖蛋白2（SGP - 2）的潜在人类同源物。在模拟内在神经元丢失和/或传入神经阻滞的实验性损伤后，大鼠海马中的SGP - 2表达增加。pADHC - 9在脑中的功能尚未明确，但在血清中，一种类似的蛋白质可抑制补体依赖性细胞溶解。pADHC - 9在阿尔茨海默病海马中表达增加可能是为延缓补体驱动的神经退行性级联反应而产生的一种代偿反应。
