Oda T, Pasinetti G M, Osterburg H H, Anderson C, Johnson S A, Finch C E
Andrus Gerontology Center, University of Southern California, Los Angeles 90089-0191.
Biochem Biophys Res Commun. 1994 Nov 15;204(3):1131-6. doi: 10.1006/bbrc.1994.2580.
Clusterin, a 70-80 kDa sulfated glycoprotein found in numerous tissues, is also known as complement lysis inhibitor (CLI), apolipoprotein J, SP-40,40, TRPM-2, and SGP-2. In Alzheimer disease (AD), clusterin mRNA is increased, whereas clusterin protein is found in deposits of beta-amyloid (A beta). These studies characterized clusterin protein from human brain. In extracts from cortex and hippocampus, clusterin was about 40% higher in AD than in controls. Purified clusterin from human brain was slightly smaller than serum clusterin. Brain and serum clusterin were indistinguishable in the inhibition of complement-mediated hemolysis. Both serum and brain clusterin were indistinguishable in inhibiting the aggregation of A beta and promoting oxidative stress in rat pheochromocytoma PC12 cells (MTT assay). The inhibition of A beta aggregation and enhancement of A beta toxicity by clusterin suggest new mechanisms in AD.
凝集素是一种在多种组织中发现的70-80 kDa硫酸化糖蛋白，也被称为补体溶解抑制剂（CLI）、载脂蛋白J、SP-40,40、TRPM-2和SGP-2。在阿尔茨海默病（AD）中，凝集素mRNA增加，而凝集素蛋白存在于β-淀粉样蛋白（Aβ）沉积物中。这些研究对来自人脑的凝集素蛋白进行了表征。在皮质和海马体的提取物中，AD患者的凝集素比对照组高约40%。从人脑中纯化的凝集素比血清凝集素略小。脑凝集素和血清凝集素在抑制补体介导的溶血方面没有区别。血清和脑凝集素在抑制Aβ聚集和促进大鼠嗜铬细胞瘤PC12细胞中的氧化应激方面（MTT试验）也没有区别。凝集素对Aβ聚集的抑制和对Aβ毒性的增强提示了AD中的新机制。
