Prabagaran Solai Ramatchandirane, Manorama Ruth, Delille Daniel, Shivaji Sisinthy
Centre for Cellular and Molecular Biology, Hyderabad, India.
FEMS Microbiol Ecol. 2007 Feb;59(2):342-55. doi: 10.1111/j.1574-6941.2006.00213.x. Epub 2006 Oct 5.
Bacterial diversity in sub-Antarctic seawater, collected off Ushuaia, Argentina, was examined using a culture independent approach. The composition of the 16S rRNA gene libraries from seawater and seawater contaminated with the water soluble fraction of crude oil was statistically different (P value 0.001). In both libraries, clones representing the Alphaproteobacteria, Gammaproteobacteria, the Cytophaga-Flavobacterium-Bacteroidetes group and unculturable bacteria were dominant. Clones associated with the genera Roseobacter, Sulfitobacter, Staleya, Glaciecola, Colwellia, Marinomonas, Cytophaga and Cellulophaga were common to both the libraries. However, clones associated with Psychrobacter, Arcobacter, Formosa algae, Polaribacter, Ulvibacter and Tenacibaculum were found only in seawater contaminated with hydrocarbons (Table 1). Further, the percentage of clones of Roseobacter, Sulfitobacter and Glaceicola was high in seawater (43%, 90% and 12% respectively) compared to seawater contaminated with hydrocarbons (35%, 4% and 9% respectively). One of the clones F2C63 showed 100% similarity with Marinomonas ushuaiensis a bacterium identified by us from the same site.
采用非培养方法对从阿根廷乌斯怀亚附近采集的亚南极海水的细菌多样性进行了研究。来自海水和被原油水溶性部分污染的海水的16S rRNA基因文库的组成在统计学上存在差异（P值为0.001）。在这两个文库中，代表α-变形菌纲、γ-变形菌纲、噬纤维菌-黄杆菌-拟杆菌群和不可培养细菌的克隆占主导地位。与玫瑰杆菌属、硫杆菌属、斯氏菌属、嗜冷杆菌属、科尔韦氏菌属、海单胞菌属、噬纤维菌属和食纤维菌属相关的克隆在两个文库中都很常见。然而，与嗜冷杆菌属、弓形杆菌属、海藻福氏菌属、极地杆菌属、绿弯菌属和黄杆菌属相关的克隆仅在被碳氢化合物污染的海水中发现（表1）。此外，与被碳氢化合物污染的海水（分别为35%、4%和9%）相比，玫瑰杆菌属、硫杆菌属和嗜冷杆菌属的克隆在海水中的百分比很高（分别为43%、90%和12%）。其中一个克隆F2C63与我们从同一地点鉴定出的乌斯怀亚海单胞菌具有100%的相似性。
