Shravage Bhupendra V, Dayananda Kannayakanahalli M, Patole Milind S, Shouche Yogesh S
Molecular Biology Unit, National Centre for Cell Science, Ganeshkhind, Pune 411007, India.
Microbiol Res. 2007;162(1):15-25. doi: 10.1016/j.micres.2006.01.005. Epub 2006 Mar 6.
The aim of our study was to estimate the uncultured eubacterial diversity of a soil sample collected below a dead seal, Cape Evans, McMurdo, Antarctica by an SSU rDNA gene library approach. Our study by sequencing of clones from SSU rDNA gene library approach revealed high diversity in the soil sample from Antarctica. More than 50% of clones showed homology to Cytophaga-Flavobacterium-Bacteroides group; sequences also belonged to alpha, beta, gamma proteobacteria, Thermus-Deinococcus and high GC gram-positive group; Phylogenetic analysis of the SSU rDNA clones showed the presence of species belonging to Cytophaga spp., Vitellibacter vladivostokensis, Aequorivita lipolytica, Aequorivita crocea, Flavobacterium spp., Flexibacter sp., Subsaxibacter broadyi, Bacteroidetes, Roseobacter sp., Sphingomonas baekryungensis, Nitrosospira sp., Nitrosomonas cryotolerans, Psychrobacter spp., Chromohalobacter sp., Psychrobacter okhotskensis, Psychrobacter fozii, Psychrobacter urativorans, Rubrobacter radiotolerans, Marinobacter sp., Rubrobacteridae, Desulfotomaculum aeronauticum and Deinococcus sp. The presence of ammonia oxidizing bacteria in Antarctica soil was confirmed by the presence of the amoA gene. Phylogenetic analysis revealed grouping of clones with their respective groups.
我们研究的目的是通过小亚基核糖体DNA（SSU rDNA）基因文库方法，估算从南极洲麦克默多埃文斯角一只海豹尸体下方采集的土壤样本中未培养真细菌的多样性。我们通过对SSU rDNA基因文库方法中的克隆进行测序研究发现，来自南极洲的土壤样本具有高度多样性。超过50%的克隆与噬纤维菌-黄杆菌-拟杆菌群具有同源性；序列还属于α、β、γ变形菌纲、栖热菌-嗜热放线菌和高GC含量革兰氏阳性菌群；对SSU rDNA克隆的系统发育分析表明，存在属于噬纤维菌属、海参威维氏菌、解脂海栖热袍菌、黄色海栖热袍菌、黄杆菌属、屈挠杆菌属、布氏亚栖热菌、拟杆菌门、玫瑰杆菌属、白陵鞘氨醇单胞菌、亚硝化螺菌属、耐冷亚硝化单胞菌、嗜冷杆菌属、嗜盐色杆菌属、鄂霍次克嗜冷杆菌、福氏嗜冷杆菌、食尿嗜冷杆菌、耐辐射红球菌、海杆菌属、红球菌科、嗜航脱硫肠状菌和嗜热放线菌属的物种。通过氨单加氧酶基因（amoA基因）的存在证实了南极洲土壤中存在氨氧化细菌。系统发育分析揭示了克隆与其各自菌群的分组情况。
