Vijaya Padma V, Shyamala Devi C S, Ramkumar K M
Department of Biochemistry and Molecular Biology, University of Madras, Chennai, India.
Nutrition. 2006 Nov-Dec;22(11-12):1171-6. doi: 10.1016/j.nut.2006.08.010. Epub 2006 Oct 4.
In the present study, the protective effect of fish oil treatment on the fatty acid composition in isoproterenol (IPH)-induced myocardial infarction was studied in male albino Wistar rats.
Rats were injected for 2 consecutive days with IPH (60 mg/kg body weight) at 24-h intervals to induce myocardial infarction. Fish oil was administered orally at a dose of 0.05 mL/d for 45 d, after which serum and heart tissue were assayed for lipid profile, lipoprotein changes, and myocardial membrane phospholipid fatty acid composition.
Biochemical assessment of myocardial infarction was done by measuring the activities of creatinine kinase and lactate dehydrogenase, which were significantly elevated in the rats administered with IPH. Further, the administration of IPH modified the fatty acid composition and analysis of fatty acids showed there was an increase in the omega-3/omega-6 ratio in phospholipid pool. In addition, increased levels of total cholesterol, free cholesterol, ester cholesterol, phospholipids, triacylglycerols and free fatty acid was observed in serum and heart tissue of IPH-induced rats. The fish oil treatment for a period of 45 d decreased the levels of cardiac markers (creatinine kinase and lactate dehydrogenase) and reversed the biochemical lesions induced by IPH.
Our study suggests that fish oil treatment has a hypolipidemic effect and has potential use in the treatment of myocardial infarction.
在本研究中，研究了鱼油处理对异丙肾上腺素（IPH）诱导的雄性白化Wistar大鼠心肌梗死中脂肪酸组成的保护作用。
大鼠每隔24小时连续2天注射IPH（60mg/kg体重）以诱导心肌梗死。以0.05mL/d的剂量口服鱼油，持续45天，之后检测血清和心脏组织的脂质谱、脂蛋白变化以及心肌膜磷脂脂肪酸组成。
通过测量肌酐激酶和乳酸脱氢酶的活性对心肌梗死进行生化评估，在给予IPH的大鼠中这些酶的活性显著升高。此外，IPH的给予改变了脂肪酸组成，脂肪酸分析表明磷脂池中ω-3/ω-6比值增加。另外，在IPH诱导的大鼠的血清和心脏组织中观察到总胆固醇、游离胆固醇、胆固醇酯、磷脂、三酰甘油和游离脂肪酸水平升高。为期45天的鱼油处理降低了心脏标志物（肌酐激酶和乳酸脱氢酶）的水平，并逆转了IPH诱导的生化损伤。
我们的研究表明，鱼油处理具有降血脂作用，在心肌梗死治疗中具有潜在用途。
