Yogeeta Surinder Kumar, Hanumantra Rao Balaji Raghavendran, Gnanapragasam Arunachalam, Senthilkumar Subramanian, Subhashini Rajakannu, Devaki Thiruvengadam
Department of Biochemistry, University of Madras, Guindy Campus, Chennai-600 025, Tamil Nadu, India.
Basic Clin Pharmacol Toxicol. 2006 May;98(5):467-72. doi: 10.1111/j.1742-7843.2006.pto_335.x.
The present study aims at evaluating the effect of the combination of ferulic acid and ascorbic acid on isoproterenol-induced abnormalities in lipid metabolism. The rats were divided into eight groups: Control, isoproterenol, ferulic acid alone, ascorbic acid alone, ferulic acid+ascorbic acid, ferulic acid+isoproterenol, ascorbic acid+isoproterenol and ferulic acid+ascorbic acid+isoproterenol. Ferulic acid (20 mg/kg b.w.t.) and ascorbic acid (80 mg/kg b.w.t.) both alone and in combination was administered orally for 6 days and on the fifth and the sixth day, isoproterenol (150 mg/kg b.w.t.) was injected intraperitoneally to induce myocardial injury to rats. Induction of rats with isoproterenol resulted in a significant increase in the levels of triglycerides, total cholesterol, free fatty acids, free and ester cholesterol in both serum and cardiac tissue. A rise in the levels of phospholipids, lipid peroxides, low density lipoprotein and very low density lipoprotein-cholesterol was also observed in the serum of isoproterenol-intoxicated rats. Further, a decrease in the level of high density lipoprotein in serum and in the phospholipid levels, in the heart of isoproterenol-intoxicated rats was observed, which was paralleled by abnormal activities of lipid metabolizing enzymes: total lipase, cholesterol ester synthase, lipoprotein lipase and lecithin: cholesterol acyl transferase. Pre-cotreatment with the combination of ferulic acid and ascorbic acid significantly attenuated these alterations and restored the levels to near normal when compared to individual treatment groups. Histopathological observations were also in correlation with the biochemical parameters. These findings indicate the synergistic protective effect of ferulic acid and ascorbic acid on isoproterenol-induced abnormalities in lipid metabolism.
本研究旨在评估阿魏酸和抗坏血酸联合使用对异丙肾上腺素诱导的脂质代谢异常的影响。将大鼠分为八组：对照组、异丙肾上腺素组、单独使用阿魏酸组、单独使用抗坏血酸组、阿魏酸+抗坏血酸组、阿魏酸+异丙肾上腺素组、抗坏血酸+异丙肾上腺素组和阿魏酸+抗坏血酸+异丙肾上腺素组。阿魏酸（20毫克/千克体重）和抗坏血酸（80毫克/千克体重）单独及联合使用，口服给药6天，在第五天和第六天，腹腔注射异丙肾上腺素（150毫克/千克体重）以诱导大鼠心肌损伤。用异丙肾上腺素诱导大鼠导致血清和心脏组织中甘油三酯、总胆固醇、游离脂肪酸、游离胆固醇和胆固醇酯水平显著升高。在异丙肾上腺素中毒大鼠的血清中还观察到磷脂、脂质过氧化物、低密度脂蛋白和极低密度脂蛋白胆固醇水平的升高。此外，观察到异丙肾上腺素中毒大鼠血清中高密度脂蛋白水平和心脏中磷脂水平降低，同时脂质代谢酶（总脂肪酶、胆固醇酯合酶、脂蛋白脂肪酶和卵磷脂：胆固醇酰基转移酶）的活性异常。与单独治疗组相比，阿魏酸和抗坏血酸联合预处理显著减轻了这些改变，并使水平恢复到接近正常。组织病理学观察结果也与生化参数相关。这些发现表明阿魏酸和抗坏血酸对异丙肾上腺素诱导的脂质代谢异常具有协同保护作用。
