Dey Ayan, Singh Sarman
Division of Clinical Microbiology, Department of Laboratory Medicine, All India Institute of Medical Sciences, New Delhi 110029, India.
Infect Genet Evol. 2007 Mar;7(2):219-22. doi: 10.1016/j.meegid.2006.09.001. Epub 2006 Oct 5.
In India and Sudan, some patients of visceral leishmaniasis develop post-Kala-Azar dermal leishmaniasis (PKDL) while majority will not. Similarly, the clinical manifestations and treatment outcome are reported to vary from district to district and state to state in India. Present study is focused on to find out the genetic variations between VL & PKDL causing strains. Nuclear DNA from 24 strains of Leishmania donovani, isolated from patients of visceral leishmaniasis (18) and PKDL (6) was extracted and digested with PstI restriction enzyme followed by southern hybridization using Dig labeled beta-tubulin as probe. The results showed three different banding patterns among 18 VL strains. However, all PKDL isolates showed a genetic homogeneity within themselves but heterogeneity from VL isolates. Interestingly maximum heterogenic groups were found in Bihar but all isolates from West Bengal showed a single genotype origin. This study shows that genetic mutations might be responsible for such variation and development of PKDL in visceral strains of Indian L. donovani.
在印度和苏丹，一些内脏利什曼病患者会发展为黑热病后皮肤利什曼病（PKDL），而大多数患者不会。同样，据报道，在印度，不同地区和邦的临床表现和治疗结果也有所不同。本研究旨在找出导致内脏利什曼病（VL）和PKDL的菌株之间的基因变异。从内脏利什曼病患者（18例）和PKDL患者（6例）中分离出24株杜氏利什曼原虫的核DNA，用PstI限制性内切酶消化，然后以地高辛标记的β-微管蛋白为探针进行Southern杂交。结果显示，18株VL菌株中有三种不同的条带模式。然而，所有PKDL分离株自身显示出基因同质性，但与VL分离株存在异质性。有趣的是，在比哈尔邦发现了最大的异源群体，但来自西孟加拉邦的所有分离株都显示出单一的基因型起源。这项研究表明，基因突变可能是印度杜氏利什曼原虫内脏菌株中PKDL这种变异和发展的原因。
