Stone W L, Reynolds J A
J Biol Chem. 1975 Oct 25;250(20):8045-8.
The major polypeptide components of human high density lipoprotein, apo-Gln-I and apo-Gln-II, self-associate at pH 8.3 and ionic strength 0.045. The dimeric (MW = 56,800) association constant for apo-Gln-I is 1.3 X 10(4)liters/mol. apo-Gln-II is tetrameric at all experimentally accessible concentrations. Self-association of apo-Gln-I is accompanied by minor conformational alterations distinct from that induced by saturating levels of bound amphiphilic ligands. These results are discussed with respect to the design of reconstitution experiments between the apoproteins and lipids.
人类高密度脂蛋白的主要多肽成分，载脂蛋白-Gln-I和载脂蛋白-Gln-II，在pH 8.3和离子强度0.045条件下会发生自缔合。载脂蛋白-Gln-I的二聚体（分子量 = 56,800）缔合常数为1.3×10⁴升/摩尔。在所有实验可及的浓度下，载脂蛋白-Gln-II都是四聚体。载脂蛋白-Gln-I的自缔合伴随着轻微的构象变化，这与由饱和水平的结合两亲性配体诱导的构象变化不同。结合载脂蛋白与脂质之间的重组实验设计对这些结果进行了讨论。
