Golovin Andrey, Spiridonova Vera, Kopylov Alexei
Department of Bioengineering and Bioinformatics, Moscow State University, 119992 Moscow, Russian Federation.
FEBS Lett. 2006 Oct 30;580(25):5858-62. doi: 10.1016/j.febslet.2006.09.050. Epub 2006 Oct 2.
In E. coli, S7 initiates 30S ribosome assembly by binding to 16S rRNA. It also regulates translation of the S12 and S7 cistrons of the 'streptomycin' operon transcript by binding to the S12-S7 intercistronic region. Here, we describe the contacts of N-terminally His(6)-tagged S7 with this region as mapped by UV-induced cross-linking. The cross-links are located at U(-34), U(-35), quite distant from the start codons of the two cistrons. In order to explain the mechanism of translational repression of S12-S7, we consider a possible conformational rearrangement of the intercistronic RNA structure induced by S7 binding.
在大肠杆菌中，S7通过与16S rRNA结合启动30S核糖体组装。它还通过与“链霉素”操纵子转录本的S12 - S7顺反子间区域结合来调节S12和S7顺反子的翻译。在此，我们描述了通过紫外线诱导交联绘制的N端带有His(6)标签的S7与该区域的接触情况。交联位于U(-34)、U(-35)处，与两个顺反子的起始密码子相距甚远。为了解释S12 - S7翻译抑制的机制，我们考虑了由S7结合诱导的顺反子间RNA结构可能发生 conformational rearrangement（构象重排）。 （注：原文中“conformational rearrangement”在中文里较难准确简洁表述，这里保留英文供参考，实际应用中可根据具体情况准确翻译或解释，比如“构象重排” ）
