Araki Kiyomi Y, Fujimura Satoshi, MacDonald Marcy E, Bhide Pradeep G
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
Dev Neurosci. 2006;28(6):518-27. doi: 10.1159/000095114.
Dopamine and its receptors appear in the developing brain early in the embryonic period and dopamine receptor activation influences proliferation and differentiation of neuroepithelial precursor cells. Since dopamine D(1) and D(2) receptor activation produces opposing effects on precursor cell activity, dopamine's overall effects may correlate with relative numbers and activity of each receptor subtype on the precursor cells. Dopamine receptor expression and activity in individual precursor cells in the intact brain are difficult to ascertain. Therefore, cell lines with known receptor expression profiles can be useful tools to study dopamine's influence on neuroepithelial cells. We report characterization of dopamine receptor expression and activity profiles in three mouse striatal precursor cell lines and suggest that these cell lines can be valuable tools to study dopamine's effects on striatal precursor cell proliferation and differentiation.
多巴胺及其受体在胚胎期早期就出现在发育中的大脑中，多巴胺受体激活会影响神经上皮前体细胞的增殖和分化。由于多巴胺D(1)和D(2)受体激活对前体细胞活性产生相反的影响，多巴胺的总体效应可能与前体细胞上每种受体亚型的相对数量和活性相关。完整大脑中单个前体细胞的多巴胺受体表达和活性难以确定。因此，具有已知受体表达谱的细胞系可能是研究多巴胺对神经上皮细胞影响的有用工具。我们报告了三种小鼠纹状体前体细胞系中多巴胺受体表达和活性谱的特征，并表明这些细胞系可能是研究多巴胺对纹状体前体细胞增殖和分化影响的有价值工具。
