de Franco Fernando Faria, Kuhn Gustavo Campos Silva, de Sene Fábio Melo, Manfrin Maura Helena
Departamento de Genética, Universidade de São Paulo-Faculdade de Medicina de Ribeirão Preto, Bloco A - Av. dos Bandeirantes, 3900, 14049-900, Ribeirão Preto, SP, Brazil.
Genetica. 2006 Sep-Nov;128(1-3):287-95. doi: 10.1007/s10709-006-6248-4.
In this study, we have compared 34 repetition units of pBuM-2 satellite DNA of individuals from six isolated populations of Drosophila gouveai, a cactophilic member of Drosophila buzzatii cluster (repleta group). In contrast to the results of previous morphological and molecular data, which suggest differentiation among the D. gouveai populations, the sequences and the cluster analysis of pBuM-2 monomers showed that this repetitive element is highly conserved among the six D. gouveai populations (97.8% similarity), indicating a slow rate of evolution of pBuM-2 sequences at the population level. Probably, some homogenization mechanisms of tandem sequences, such as unequal crossing or gene conversion, have maintained the sequence similarity of pBuM-2 among D. gouveai populations. Alternatively, such a result may be associated with a functional role of pBuM-2 sequences, although it is not understood at present.
在本研究中，我们比较了果蝇属戈韦氏果蝇六个隔离种群个体的pBuM - 2卫星DNA的34个重复单元，戈韦氏果蝇是果蝇布扎蒂集群（repleta组）的嗜仙人掌成员。与之前形态学和分子数据表明戈韦氏果蝇种群间存在分化的结果相反，pBuM - 2单体的序列和聚类分析表明，这种重复元件在六个戈韦氏果蝇种群中高度保守（相似度为97.8%），这表明pBuM - 2序列在种群水平上的进化速率较慢。可能是一些串联序列的均一化机制，如不等交换或基因转换，维持了戈韦氏果蝇种群间pBuM - 2的序列相似性。另外，这样的结果可能与pBuM - 2序列的功能作用有关，尽管目前尚不清楚。
