Hassell J A, Colby C, Romano A H
J Cell Physiol. 1975 Aug;86(1):37-45. doi: 10.1002/jcp.1040860106.
Serum starvation of growing and nongrowing (density-inhibited) mouse 3T3 cells resulted in decreased phosphorylation of 2-deoxy--D-glucose, while the time course of transport of this sugar remained unchanged. Serum starvation of SV40 transformed 3T3 cells (SV101) and spontaneously transformed 3T6 cells did not alter either the time course of transport, or phosphorylation of the sugar. Treatment of SV101 cells with 10(-4) M dibutyryl adenosine cyclic 3':5' monophosphate and 10(-3) M theophylline did not restore the capacity to regulate 2-deoxy-D-glucose phosphorylation when these cells were serum deprived. We conclude that serum factors are involved in the modulation of phosphorylation of 2-deoxy-D-glucose in 3T3 cells rather than its transport. This regulation is operative both in growing as well as nongrowing 3T3 cells. In contrast, transformed cells do not respond to this regulation of 2-deoxy-D-glucose phosphorylation.
对生长中的和非生长状态（密度抑制）的小鼠3T3细胞进行血清饥饿处理，导致2-脱氧-D-葡萄糖的磷酸化减少，而这种糖的转运时间进程保持不变。对SV40转化的3T3细胞（SV101）和自发转化的3T6细胞进行血清饥饿处理，既未改变糖的转运时间进程，也未改变其磷酸化。当用10⁻⁴ M二丁酰腺苷环3':5'-单磷酸和10⁻³ M茶碱处理SV101细胞时，在这些细胞血清剥夺的情况下，并未恢复调节2-脱氧-D-葡萄糖磷酸化的能力。我们得出结论，血清因子参与调节3T3细胞中2-脱氧-D-葡萄糖的磷酸化，而非其转运。这种调节在生长中的和非生长状态的3T3细胞中均起作用。相比之下，转化细胞对2-脱氧-D-葡萄糖磷酸化的这种调节没有反应。
