Barnes D W, Brown V T, Colowick S P
J Cell Physiol. 1978 Nov;97(2):231-9. doi: 10.1002/jcp.1040970212.
Addition of 5 to 250 micromolar adenosine to the culture medium resulted in a 30-80% inhibition of the rate of uptake of 2-deoxyglucose or 3-0-methylglucose by sparse or confluent 3T3 cells within three hours. The inhibition of deoxyglucose uptake could be reversed partially by changing the cells to medium without adenosine for two hours and could be prevented completely by the addition of persantin, an inhibitor of nucleoside uptake. The adenosine effect is not due to inhibition of pyrimidine synthesis, since it is not prevented by uridine. It is not seen in 3T6 cells lacking adenosine kinase. The inhibition could be observed on confluent cells whose deoxyglucose uptake was stimulated by insulin, epidermal growth factor (EGF), calf serum or calcium phosphate. Although the percentage stimulation over control by these factors varied, the percentage inhibition by addition of adenosine of the stimulated rates, as well as the unstimulated rate, was relatively constant. EGF, insulin and calcium phosphate caused little or no stimulation of deoxyglucose uptake by sparse cells, whether adenosine treated or untreated. The results suggest that adenosine acts intracellularly after phosphorylation to regulate sugar uptake through a mechanism which is independent of the regulation by hormones and cell density.
向培养基中添加5至250微摩尔的腺苷，会导致稀疏或汇合的3T3细胞在三小时内对2-脱氧葡萄糖或3-0-甲基葡萄糖的摄取速率产生30%至80%的抑制。将细胞更换为不含腺苷的培养基两小时，可部分逆转对脱氧葡萄糖摄取的抑制，而添加核苷摄取抑制剂潘生丁则可完全防止这种抑制。腺苷的作用并非由于嘧啶合成受到抑制，因为尿苷并不能阻止这种作用。在缺乏腺苷激酶的3T6细胞中未观察到这种抑制作用。在汇合细胞中可以观察到这种抑制作用，这些汇合细胞的脱氧葡萄糖摄取受到胰岛素、表皮生长因子（EGF）、小牛血清或磷酸钙的刺激。尽管这些因素对对照组的刺激百分比各不相同，但添加腺苷对刺激速率以及未刺激速率的抑制百分比相对恒定。无论是否用腺苷处理，EGF、胰岛素和磷酸钙对稀疏细胞的脱氧葡萄糖摄取几乎没有或没有刺激作用。结果表明，腺苷在磷酸化后在细胞内起作用，通过一种独立于激素和细胞密度调节的机制来调节糖摄取。
