Jiang Wen-xi, Qiu Chang-chun, Cheng Zu-heng, Niu Wen-quan
Xinjiang Medical University, Urumqi, Xinjiang, 830054 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2006 Oct;23(5):523-7.
To investigate the association of polymorphisms in the apolipoprotein B gene (APOB) 3'variable number of tandem repeat with natural longevity in the Xinjiang Uighur nationality people.
Totally 191 healthy individuals over 90 years and 53 individuals aged 65-70 years were recruited among Xinjiang Uighur population, the nationality, gender and living area were matched. Genotyping was performed using polymerase chain reaction-sequence specific primer(PCR-SSP) and PCR-sequencing.
Fourteen alleles were found in the Xinjiang Uighur nationality population. The frequency of HVE36 and HVE42 in the natural longevity group were significantly higher than that in the control group (both P<0.05) and HVE44, HVE46, HVE48 and HVE58 were only found in the natural longevity group. However, the frequency of HVE26, HVE30 and HVE34 were markedly lower in the natural longevity group compared to the control group. Logistic regression analyses revealed that allele L and the genotypes LL were positively associated with age, whereas the allele S and genotype SS were negatively associated with age (both P<0.05). Each allele consists of 15 bp tandem repeats with rich-AT by PCR-sequencing.
These results indicate that the S allele, and SS genotype are frail factors in China Uighur natural longevity people, whereas allele L and genotypes LL are protective factors.
探讨载脂蛋白B基因（APOB）3'端串联重复序列多态性与新疆维吾尔族人群自然长寿的关系。
在新疆维吾尔族人群中招募191名90岁以上健康个体和53名65 - 70岁个体，民族、性别和居住地区相匹配。采用聚合酶链反应 - 序列特异性引物（PCR - SSP）和PCR测序进行基因分型。
在新疆维吾尔族人群中发现了14个等位基因。自然长寿组中HVE36和HVE42的频率显著高于对照组（均P<0.05），且HVE44、HVE46、HVE48和HVE58仅在自然长寿组中发现。然而，自然长寿组中HVE26、HVE30和HVE34的频率明显低于对照组。逻辑回归分析显示，等位基因L和基因型LL与年龄呈正相关，而等位基因S和基因型SS与年龄呈负相关（均P<0.05）。通过PCR测序可知，每个等位基因由富含AT的15bp串联重复序列组成。
这些结果表明，在中国维吾尔族自然长寿人群中，等位基因S和基因型SS是脆弱因素，而等位基因L和基因型LL是保护因素。
