Peng Xiaomei, Wupuer Hamulati, Maitiyusup Dolkun, Kurban Palida, Dai Wencheng, Mamat Tursun
College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
Wei Sheng Yan Jiu. 2010 Mar;39(2):197-200.
This study was to investigate the relationship between the 40 bp VNTR polymorphism of dopamine transporter gene (DAT1) and cancer with Uighur medicine abnormal Hilit on Chinese Uighur population of Xinjiang province.
Divided the cancer patients into four body fluids according to Uighur medicine theory, the polymerase chain reaction and VNTR polymorphism technique was employed to detect genotype and allele frequencies of a 40 bp VNTR polymorphism situated in 3' untranslated region of the DAT1 gene in 47 cancer patients with abnormal black Savda, 26 cancer patients with other abnormal Hilit and 57 normal control subjects in Uighur population of Xinjiang Province.
(1) In our sample,the repeat numbers of 40 bp were 6 to 11 (PCR product length of 320 bp to 520 bp) and 10-repeats allele (480 bp) detected was the dominant allele of DAT1 gene polymorphisms with the allele frequency 90.4%. Six kinds of genotype were detected in this study and the genotype 480 bp/480 bp was the most common genotype with genotype frequency 80.7%. (2) The susceptibility to cancer patients with abnormal black Savda among the subjects with 10 repeat (R) allele and 10/10 genotypes and the subjects with non-10 repeat (R) allele and non-10/10 genotypes were similar (P = 0.158, OR = 1.994, 95% CI = 0.754-5.275; P = 0.138, OR = 2.143, 95% CI = 0.772-5.947). No significant differences for the genotype frequency or the allele frequency of the 40 bp VNTR polymorphism of DAT1 were revealed between cancer patients with abnormal black Savda and cancer patients with other abnormal Hilit (P = 0.729, P = 0.782).
The 40 bp VNTR polymorphism of DAT1 may not be correlated to the susceptibility to cancer with Uighur medicine abnormal Hilit.
本研究旨在探讨多巴胺转运体基因（DAT1）40bp可变数目串联重复序列（VNTR）多态性与新疆维吾尔族人群中维吾尔医异常体液型癌症的关系。
根据维吾尔医理论将癌症患者分为四种体液类型，采用聚合酶链反应和VNTR多态性技术，检测新疆维吾尔族人群中47例异常黑胆质型癌症患者、26例其他异常体液型癌症患者及57例正常对照者DAT1基因3'非翻译区40bp VNTR多态性的基因型和等位基因频率。
（1）在本研究样本中，40bp重复次数为6至11次（PCR产物长度为320bp至520bp），检测到的10次重复等位基因（480bp）是DAT1基因多态性的优势等位基因，等位基因频率为90.4%。本研究共检测到六种基因型，其中480bp/480bp基因型最为常见，基因型频率为80.7%。（2）10次重复（R）等位基因和10/10基因型个体与非10次重复（R）等位基因和非10/10基因型个体中，异常黑胆质型癌症患者的易感性相似（P = 0.158，OR = 1.994，95%CI = 0.754 - 5.275；P = 0.138，OR = 2.143，95%CI = 0.772 - 5.947）。异常黑胆质型癌症患者与其他异常体液型癌症患者之间，DAT1基因40bp VNTR多态性的基因型频率和等位基因频率无显著差异（P = 0.729，P = 0.782）。
DAT1基因40bp VNTR多态性可能与维吾尔医异常体液型癌症易感性无关。
